A kind of preparation method of loaded interferon microcapsule
A technology of interferon and microcapsules, applied in the field of biomedicine, can solve the problems of short half-life, small molecular weight of interferon, drug resistance and side effects, etc., and achieve the effect of controllable size and shape, adjustable wall thickness and composition
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Embodiment 1
[0014] Example 1: Take the following steps:
[0015] A) Na 2 CO 3 and Ca(NO 3 ) 2 4H 2 O was respectively prepared into a solution with a concentration of 0.05 mol / L; 400mL Ca(NO 3 ) 2 Pour the solution into a beaker; then add 100mL of α-interferon into the beaker, stir well and let stand for 15 min;
[0016] B) quickly add 400mL Na to the solution obtained in step A) 2 CO 3 solution, sonicated for 10s, and allowed to stand for 15 minutes to obtain interferon-containing calcium carbonate colloidal particles; the obtained calcium carbonate colloidal particles were quickly collected by centrifugation or membrane filtration, washed three times with absolute ethanol, and dried in an oven at 50°C. Microencapsulated CaCO 3 template;
[0017] C) Take 300mg of calcium carbonate colloidal particles and place them in a 15mL centrifuge tube, shake the centrifuge tube slightly to disperse the calcium carbonate particles, wash with deionized water three times, centrifuge at 3000 ...
Embodiment 2
[0021] Embodiment 2: Take the following steps:
[0022] A) Na 2 CO 3 and Ca(NO 3 ) 2 4H 2 O was prepared into a solution with a concentration of 0.05 mol / L; take 300mL Ca(NO 3 ) 2 Pour the solution into a beaker; then add 100mL of α-interferon into the beaker, stir well and let stand for 15 min;
[0023] B) quickly add 300mL Na to the solution obtained in step A) 2 CO 3 solution, sonicated for 10s, and allowed to stand for 15 minutes to obtain interferon-containing calcium carbonate colloidal particles; the obtained calcium carbonate colloidal particles were quickly collected by centrifugation or membrane filtration, washed three times with absolute ethanol, and dried in an oven at 50°C. Microencapsulated CaCO 3 template;
[0024] C) Take 300mg of calcium carbonate colloidal particles and place them in a 15mL centrifuge tube, shake the centrifuge tube slightly to disperse the calcium carbonate particles, wash with deionized water three times, centrifuge at 3000 r / min...
Embodiment 3
[0028]Embodiment 3: Take the following steps:
[0029] A) Na 2 CO 3 and Ca(NO 3 ) 2 4H 2 O was prepared into a solution with a concentration of 0.05 mol / L; 200 mL of Ca(NO 3 ) 2 Pour the solution into a beaker; then add 100mL of α-interferon into the beaker, stir well and let stand for 15 min;
[0030] B) quickly add 200mL Na to the solution obtained in step A) 2 CO 3 solution, sonicated for 10s, and allowed to stand for 15 minutes to obtain interferon-containing calcium carbonate colloidal particles; the obtained calcium carbonate colloidal particles were quickly collected by centrifugation or membrane filtration, washed three times with absolute ethanol, and dried in an oven at 50°C. Microencapsulated CaCO 3 template;
[0031] C) Take 300mg of calcium carbonate colloidal particles and place them in a 15mL centrifuge tube, shake the centrifuge tube slightly to disperse the calcium carbonate particles, wash with deionized water three times, centrifuge at 3000 r / min f...
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