A kind of preparation method of loaded interferon microcapsule

A technology of interferon and microcapsules, applied in the field of biomedicine, can solve the problems of short half-life, small molecular weight of interferon, drug resistance and side effects, etc., and achieve the effect of controllable size and shape, adjustable wall thickness and composition

Active Publication Date: 2016-09-14
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ordinary interferon has the disadvantages of small molecular weight, short half-life, wide system distribution and high renal clearance rate, so it is easy to produce drug resistance and other toxic side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Take the following steps:

[0015] A) Na 2 CO 3 and Ca(NO 3 ) 2 4H 2 O was respectively prepared into a solution with a concentration of 0.05 mol / L; 400mL Ca(NO 3 ) 2 Pour the solution into a beaker; then add 100mL of α-interferon into the beaker, stir well and let stand for 15 min;

[0016] B) quickly add 400mL Na to the solution obtained in step A) 2 CO 3 solution, sonicated for 10s, and allowed to stand for 15 minutes to obtain interferon-containing calcium carbonate colloidal particles; the obtained calcium carbonate colloidal particles were quickly collected by centrifugation or membrane filtration, washed three times with absolute ethanol, and dried in an oven at 50°C. Microencapsulated CaCO 3 template;

[0017] C) Take 300mg of calcium carbonate colloidal particles and place them in a 15mL centrifuge tube, shake the centrifuge tube slightly to disperse the calcium carbonate particles, wash with deionized water three times, centrifuge at 3000 ...

Embodiment 2

[0021] Embodiment 2: Take the following steps:

[0022] A) Na 2 CO 3 and Ca(NO 3 ) 2 4H 2 O was prepared into a solution with a concentration of 0.05 mol / L; take 300mL Ca(NO 3 ) 2 Pour the solution into a beaker; then add 100mL of α-interferon into the beaker, stir well and let stand for 15 min;

[0023] B) quickly add 300mL Na to the solution obtained in step A) 2 CO 3 solution, sonicated for 10s, and allowed to stand for 15 minutes to obtain interferon-containing calcium carbonate colloidal particles; the obtained calcium carbonate colloidal particles were quickly collected by centrifugation or membrane filtration, washed three times with absolute ethanol, and dried in an oven at 50°C. Microencapsulated CaCO 3 template;

[0024] C) Take 300mg of calcium carbonate colloidal particles and place them in a 15mL centrifuge tube, shake the centrifuge tube slightly to disperse the calcium carbonate particles, wash with deionized water three times, centrifuge at 3000 r / min...

Embodiment 3

[0028]Embodiment 3: Take the following steps:

[0029] A) Na 2 CO 3 and Ca(NO 3 ) 2 4H 2 O was prepared into a solution with a concentration of 0.05 mol / L; 200 mL of Ca(NO 3 ) 2 Pour the solution into a beaker; then add 100mL of α-interferon into the beaker, stir well and let stand for 15 min;

[0030] B) quickly add 200mL Na to the solution obtained in step A) 2 CO 3 solution, sonicated for 10s, and allowed to stand for 15 minutes to obtain interferon-containing calcium carbonate colloidal particles; the obtained calcium carbonate colloidal particles were quickly collected by centrifugation or membrane filtration, washed three times with absolute ethanol, and dried in an oven at 50°C. Microencapsulated CaCO 3 template;

[0031] C) Take 300mg of calcium carbonate colloidal particles and place them in a 15mL centrifuge tube, shake the centrifuge tube slightly to disperse the calcium carbonate particles, wash with deionized water three times, centrifuge at 3000 r / min f...

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PUM

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Abstract

The invention discloses a method for preparing interferon-loaded microcapsules. The method comprises the following steps: A) respectively preparing Na2CO3 and Ca(NO3)2.4H2O into solutions, and adding the Ca(NO3)2 solution into alpha interferon; B) adding the Na2CO3 solution into the solution, performing ultrasonic treatment, collecting the product by a centrifugal process, and washing and drying to obtain a microcapsule template; C) washing particles for three times, removing the supernatant by centrifugation after washing at each time, and adding bovine serum albumin into the CaCO3 template to form a bovine serum albumin layer; D) adding a crosslinking agent into the bovine serum albumin layer on the surfaces of particles of the CaCO3 template, crosslinking, removing the supernatant by centrifugation and washing for three times; E) denucleating the colloid particles of the nuclear shell structure by 0.02 M EDTA with pH of 7 to completely remove calcium carbonate; F) adding an NaBH4 solution into the microcapsules, reacting at the room temperature, removing the supernatant by centrifugation and washing for three times to obtain the interferon-loaded bovine serum albumin microcapsules. By adopting the method disclosed by the invention, microcapsules are designed and constructed, which are stable at the outside of cells and are degradable under response stimulation in the cells.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing smart and degradable microcapsules loaded with interferon. Background technique [0002] Hepatitis B is a serious infectious disease caused by hepatitis B virus (HBV), and it is also an important pathogenic factor leading to liver cancer. There are 350-400 million hepatitis B virus patients in the world, about one-third of them are Chinese patients. Every year, about 300,000 people die from HBV infection-related diseases, of which 180,000 people die from liver cancer. At present, drugs for the treatment of HBV infection are mainly divided into two categories: antiviral drugs (nucleoside analogs) and immunomodulatory drugs (interferon). Among them, interferon (IFN) is an active protein (mainly glycoprotein) with multiple functions, a cytokine produced by monocytes and lymphocytes, and has broad-spectrum anti-virus on the same kind of cells, Affect cell growth an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K38/21A61P1/16A61P31/20
Inventor 王秉刘意
Owner ZHEJIANG SCI-TECH UNIV
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