Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Expression promoter STA4 of rice stamens and lodicules and application thereof

A promoter and stamen technology, which is applied to the expression promoter STA4 of rice stamens and sap and its application fields, can solve the problems of insufficiency, not many in quantity and variety, and no specific expression promoter has been developed, so as to improve growth. Characteristics and mechanisms, the effect of increasing expression, and reducing biological metabolic costs

Active Publication Date: 2015-01-21
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number and types of tissue-specific promoters of rice that people have obtained are not many, and it is difficult to meet the strong needs of current scientific research and production, and from the inventor's understanding based on existing reports, people have not yet developed A promoter capable of specific expression in rice stamens and lodicules

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Expression promoter STA4 of rice stamens and lodicules and application thereof
  • Expression promoter STA4 of rice stamens and lodicules and application thereof
  • Expression promoter STA4 of rice stamens and lodicules and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0038] The present invention utilizes mature Nipponbare seeds (preserved by the Biotechnology Laboratory of the Rice Institute of Anhui Academy of Agricultural Sciences) to isolate promoter fragments.

[0039] The following is a brief description of the main medicinal raw materials and reagent materials used in the examples. Unless otherwise specified, the raw materials and reagents are all commercially available products.

[0040] Sodium hypochlorite (NaClO, available chlorine concentration 4%), Tween20 was purchased from Sigma Company. Hygromycin B was purchased from Roche; PEASY-Tsimple and DNA marker-Trans2K were purchased from Transgen; restriction enzymes were purchased from NEB; KOD high-fidelity polymerase ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an expression promoter of rice stamens and lodicules. The expression promoter comprises a nucleotide sequence as shown in the sequence table SEQ ID No 1 or 2 or a derivative thereof. The invention further provides a recombinant expression vector, an expression cassette and host bacteria, which are constructed by using the promoter. The expression of an exogenous gene in the rice stamens and lodicules can be driven by linking the expression promoter of rice stamens and lodicules to a gene to be expressed and transferring the gene into rice plants by a vector. The promoter specifically expressed in stamens and lodicules may be a useful tool in the research of rice fertilization. With the development of molecular biology and developmental biology, the demand for such a specific expression promoter becomes strong increasingly, and the expression promoter of rice stamens and lodicules just meets the demand. The invention has a huge potential commercial value in the cultivation of male-sterile plants, breeding and other aspects, especially.

Description

technical field [0001] The present invention relates to a promoter isolated from plants, in particular to a stamen and lodicule-specific expression promoter isolated from rice, and the present invention also relates to a recombinant expression vector and a host cell containing the stamen and lodicule-specific expression promoter As well as the application in improving plant traits, cultivating new plant varieties, etc., it belongs to the field of separation and application of plant tissue or organ-specific expression promoters. Background technique [0002] With the development of genetic transformation technology, genetic engineering has become an important means of gene function research and crop genetic improvement. As an important part of the expression vector in genetic engineering, the promoter's selection and application determine the expression of the target gene in time and space. Therefore, cloning and in-depth study of the structure of the promoter is of great he...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/82C12N1/21A01H5/00
Inventor 魏鹏程杨剑波李浩马卉李莉秦瑞英杨亚春许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com