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Gene group evaluating breast cancer molecule typing and detection kit thereof

A detection kit and molecular typing technology, applied in the direction of recombinant DNA technology, microbial determination/testing, DNA/RNA fragments, etc., can solve the sensitivity and specificity of the evaluation method detection to be verified, breast cancer molecular typing Limitation of gene groups, high cost of patients, etc., to achieve the effect of improving detection ability and efficiency, reducing detection cost, and strengthening rationality and accuracy

Active Publication Date: 2015-01-21
SHANGHAI PRECISION DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is that the current method for evaluating the molecular genotype group of breast cancer is relatively limited, especially the research on the evaluation of the molecular genotype group of breast cancer for the Asian population and the Chinese population is very limited, and the existing evaluation methods The sensitivity and specificity of detection need to be verified, and the high cost is too high for patients. It provides a set of genotypes for evaluating breast cancer molecular typing and its detection kits

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  • Gene group evaluating breast cancer molecule typing and detection kit thereof
  • Gene group evaluating breast cancer molecule typing and detection kit thereof
  • Gene group evaluating breast cancer molecule typing and detection kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Evaluating the screening of breast cancer molecular typing genogroups

[0033] experimental method:

[0034] The gene expression and clinical variables of 2034 breast cancer tumors from 14 European and American cohort studies were analyzed by EPIG gene expression profiling program, and the molecular genotype group of breast cancer was screened and evaluated. All cohort study data are published, uploaded and stored in public databases. Among them, there is 1 item in the ArrayExpress database, numbered E-TABM-158, and 13 items in the GEO database, whose numbers are GSE11121, GSE12093, GSE1456, GSE2034, GSE2603, GSE3494, GSE45255, GSE4922, GSE5327, GSE6532, GSE7390, GSE8193 and GSE9195.

[0035] The analysis consisted of two parts: 1. Unsupervised cluster analysis to identify expression profiles and associated genes, and 2. Stability analysis of candidate genes. Firstly, the correlation coefficient of each gene in 2034 samples was calculated by cluster analysis...

Embodiment 2

[0044] Example 2 Evaluating the screening of breast cancer molecular typing genogroups

[0045] The expression levels of 216 genes screened in Example 1 in fresh tumor tissues or paraffin-embedded tumor tissues of 200 Chinese breast cancer patients were detected by using DASL-seq technology and MiSeq instrument next-generation sequencing. And by comparing the prediction efficiency and biological significance, a genogroup of 60 genes including immune-related genes was finally determined to evaluate the breast cancer molecular typing genotype, which was used as the basis for further breast cancer typing. The 216 pairs of primers for the 216 genes screened in the detection example 1 are shown in Table 2, and some nucleotide sequences are shown in SEQ ID No.1 to SEQ ID No.10 in the sequence table.

[0046] Table 2 The primers of 216 genes for evaluating the molecular subtyping of breast cancer

[0047]

[0048]

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[0050]

[0051]

[0052]

[0053]

[...

Embodiment 3

[0063] Example 3 Carrying out Breast Cancer Typing by Evaluating Breast Cancer Molecular Typing Genomes

[0064] Take the breast cancer tumor tissue, extract the RNA in the tumor cells (Roche Catalog Number #3270289001), and use the general Bowti method to quantitatively analyze the gene group expression profiles of the 60 evaluation genes screened in Example 2 MiSeq raw data , and then use the SSP method of Hu et al. (Hu Z et al., A compact VEGF signature associated with distant metastases and poor outcomes. BMC Med2009, 7:9.) to perform typing and recurrence risk determination on breast cancer tumor samples.

[0065] Experimental results:

[0066] The test result report is shown in Table 4.

[0067] Table 4. Breast cancer typing and cancer recurrence risk determination using genogroups for assessing breast cancer molecular typing.

[0068]

[0069]

[0070] Breast cancer typing using genogroups that assess breast cancer molecular typing allows the classification of b...

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Abstract

The invention discloses a gene group evaluating breast cancer molecule typing and a detection kit thereof. The gene group evaluating breast cancer molecule typing is a gene group composed of 216 genes. The invention also discloses the detection kit for the gene group evaluating breast cancer molecule typing. The detection kit comprises a primer capable of detecting the genes in the gene group for evaluating breast cancer molecule typing and cancer recurrence risk. An Illumina second-generation sequencing technology DASL-Seq is employed for detecting expression spectrum of cancer tissue, and on the above basis, breast cancer cases of Chinese women are divided into five molecule typing: 1) luminal type A, 2) luminal type B, 3) HER2 enrichment type, 4) basal cell type and 5) normal-cell like type, and propagation and immunization index are combined for calculating recurrence risk and pridicting recurrence possibility in 10 years, and thus individual treatment on breast cancer is guided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a group of genogroups for assessing molecular typing of breast cancer and a detection kit thereof. Background technique [0002] The incidence of breast cancer in the urban population has been on the rise since the late 1980s, and has gradually become the most threatening malignant tumor to urban women. The latest survey results show that: At present, there are more than 4,500 new breast cancer patients in Shanghai every year, and the incidence rate ranks first in the country. One out of every 300 women in Shanghai suffers from breast cancer. As far as the whole country is concerned, there are about 300,000 new cases of female breast cancer every year, and the proportion is increasing by 2-3% every year. Data from the United States in 2013 showed that the survival rate of stage 1 breast cancer reached 100%, that of stage 2 reached 93%, and that of stage 3 reached 72%. D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6869C12Q2531/113
Inventor 周彤
Owner SHANGHAI PRECISION DIAGNOSTICS CO LTD
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