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Seal applied to scratch test of cell

A scratch test and scratch technology, applied in the field of seals, can solve the problems of cell detachment and large subjective randomness.

Inactive Publication Date: 2015-01-28
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is a lot of subjective randomness in the operation, and there is a lot of subjectivity in the position (coordinate positioning) of each scratch on the bottom surface of the cell culture plate: the number of scratches, the distance, angle and intersection between scratches tend to be large deviation
The scratching operation makes the cells adjacent to the scratched place subject to horizontal force, which easily leads to cell detachment

Method used

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  • Seal applied to scratch test of cell
  • Seal applied to scratch test of cell
  • Seal applied to scratch test of cell

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Experimental program
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Embodiment Construction

[0024] The present invention will be further described below in conjunction with accompanying drawing.

[0025] see figure 1 and figure 2 , one end of the handle 1 is provided with a chassis 2, the bottom surface of the chassis 2 is a flat bottom surface 3, and a scratch strip 4 is provided on the flat bottom surface 3, the width of the scratch strip 4 is 1 mm to 2 mm, and the depth is 3 mm to 6 mm , the edge of the scratch strip 4 is smooth, ensuring that the edge of the formed imprint is smooth. Specifically, the scratch strip 4 is a scratch strip arranged on one meridian line and equally bisects the bottom surface 3 of the plane.

[0026] Furthermore, the other end of the handle 1 is provided with a round handle 5, the round handle 5 is spherical, with a diameter of 3.5 cm, and the surface is anti-slip, which is easy to grasp and will not easily fall off from the operator's hand. Arrows are marked on the round handle 5, and the arrows are parallel to the scratch strips ...

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Abstract

The invention discloses a seal applied to a scratch test of a cell. The seal comprises a handle (1); a bottom plate (2) is arranged at one end of the handle (1); the bottom surface of the bottom plate (2) is a plane bottom surface (3); a scratch strip (4) is arranged in the plane bottom surface (3); the scratch strip (4) is 1 to 2mm in width and 3 to 6mm in depth; a circular handle (5) is arranged at the other end of the handle (1). The seal applied to the scratch test of the cell is sterile, standard, simple and easy to be used.

Description

technical field [0001] The invention relates to a stamp, in particular to a stamp used for scratch test on cells. Background technique [0002] In cell biology research on cells, it is often necessary to do a scratch test on the cells cultured in the cell culture plate to detect their diffusion ability; but there is no special sterile, standardized, and easy-to-use scratch test Tools for experimentation. The current common method is to connect the pipette gun to the 10ul pipette tip and hold it tightly with one hand, fix the cell culture plate with the other hand, cooperate with both hands, and draw the pipette tip quickly and straightly under the gaze of the eyes. Make a scratch on the bottom of the cell culture plate. This method of scratch test has high requirements for the operator's hand-eye coordination and hand-eye coordination, and the operator must have a considerable amount of operating experience to carry out. In the process of operation, irregular scratches ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B41K1/02G01N13/00
Inventor 代贺龙彭龙开彭风华余少杰林旻洁谢续标卜迟斌
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV