Freezing and unfreezing method for boar semen

A technology of porcine semen and semen, which is applied in the field of freezing and thawing of porcine semen, to achieve the effect of improving utilization rate, wide application prospect, and great genetic progress

Inactive Publication Date: 2015-02-04
SHANDONG XINJI ANIMAL HUSBANDRY
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Problems solved by technology

However, in my country's pig production, the work carried out on the research and application of frozen semen is still lacking. Therefore, in-depth research and application of this technology is not only of great significance for furt...

Method used

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Embodiment Construction

[0019] Below in conjunction with specific embodiment, the present invention is described in further detail, but implementation method of the present invention is not limited to the scope that embodiment shows, and this embodiment only limits to illustrate the present invention, and is not intended to limit the scope of the present invention.

[0020] A method for freezing and thawing pig semen, comprising the following steps:

[0021] (1), the freezing process of pig semen:

[0022] ① First collect the semen of excellent boars, measure the internal sperm density with a sperm density meter, and then dilute the semen with a diluent at a temperature of 37-40°C to make the ratio of the final number of viable sperm to the whole boar semen After dilution, the semen was equilibrated at room temperature at 17-20°C for 0.5-1h.

[0023] ② Transfer the semen in the above ① into an environment of 15°C to cool down, and equilibrate for 3 hours.

[0024] ③Take 800g of the semen in ② above...

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Abstract

The invention discloses a freezing and unfreezing method for boar semen. The method includes the steps of: (1) collecting excellent breeding boar semen, conducting semen detection, then carrying out dilution in proportion, and then performing balancing for 0.5-1h; (2) transferring the semen into a 15DEG C condition, and carrying out cooling balancing for 3h; (3) conducting centrifugation under a 15DEG C condition (800g, 10min), adding a glycerol-free I solution, slowly lowering the temperature to 5DEG C, performing balancing for 2h, then adding a glycerol-containing II solution, and then conducting subpackaging; (4) carrying out the freezing procedure, then putting the product into liquid nitrogen for preservation; (5) carrying out unfreezing on the contrary to the freezing principle; and (6) detecting the quality of the unfrozen semen. With the method provided by the invention, the semen can be preserved for a long time, the utilization rate of boars is effectively improved, the cost of living body breed conservation is reduced, excellent gene drift and infectious diseases can be prevented, thus facilitating in-depth development of pig joint breeding so as to achieve greater genetic progress. The method provided by the invention has important research value and wide application prospects in protection of local excellent varieties, acceleration of breed improvement and other aspects.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation of pig semen, in particular to a freezing and thawing method of pig semen. Background technique [0002] The artificial insemination (AI) technology of pigs is the use of artificial methods to collect semen from boars, perform quality inspection, dilution and storage, etc., and then use equipment to transport the semen to the reproductive organs of estrus sows to impregnate them . Artificial insemination can improve the breeding performance of boars. Compared with the original cross, the number of sows mated by an excellent boar can be increased by 10 to 20 times per year during artificial insemination. Due to the increase in the utilization rate of fine-bred boars, the improvement of breeds has been accelerated, which can bring huge benefits to pig production and pig breeding; reduce the number of boars raised, and save feeding and management costs; it can overcome the difficulties of n...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 吴衍岭陈宪彬刘晶乔富兴彭兴泉杨军峰袁震
Owner SHANDONG XINJI ANIMAL HUSBANDRY
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