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Reagent and detection method capable of specifically detecting mia antigen gene

A technology of antigen gene and detection method, which is applied in the direction of biochemical equipment and methods, microbe determination/inspection, etc., and can solve the problem of no Mia antigen-specific gene detection products, etc.

Active Publication Date: 2016-08-24
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there are no genetic testing products specific for Mia antigen in the world or in China

Method used

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  • Reagent and detection method capable of specifically detecting mia antigen gene
  • Reagent and detection method capable of specifically detecting mia antigen gene
  • Reagent and detection method capable of specifically detecting mia antigen gene

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Embodiment Construction

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0020] Selected reagents: The kit includes 250 μL of 2× master mix, 1 mL of RNase / DNase-free water, 25 μL of Mia upstream primer at a concentration of 10 μM / L, 25 of Mia downstream primer at a concentration of 10 μM / L, 25 μL internal reference upstream primer with a concentration of 10 μM / L, 25 μL internal reference downstream primer with a concentration of 10 μM / L, and 25 μL positive control.

[0021] The 2× master mix is ​​a product of Inshore Protein Technology Co., Ltd., and its components are composed of Taq DNA...

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Abstract

The invention discloses a reagent and detection method capable of specifically detecting Mia antigen gene, the reagent is composed of 2 * premixed liquid, a Mia upstream primer, a Mia downstream primer, an internal reference upstream primer, an internal reference downstream primer, and DNA enzyme / RNA enzyme-free water, when in use, blood genome and the reagent are mixed for PCR (polymerase chain reaction) amplification reaction, PCR products are identified by electrophoresis of. According to the reagent and detection method capable of specifically detecting the Mia antigen gene, a Mia antigen gene specific primer is obtained through design, the simple, rapid, sensitive and specific detection method is established by use of the Mia antigen gene specific primer, the restrictions of a traditional serological detection method is broken through, and the method is conducive to large-scale automated screening for the Mia antigen, and can provide effective reference for clinical Mia antigen detection and identification and blood matching work.

Description

technical field [0001] The invention relates to molecular biology detection reagents and methods, in particular to a specific gene detection reagent and method for Mia antigen in Miltenberger blood group system. Background technique [0002] In 1927, Landsteiner and Levine discovered the human MNS blood group. The number of polymorphisms in the antigens of this blood group system is second only to that of the Rh blood group system. The Miltenberger blood group system is a relatively rare subsystem in the MNS system. It is a group of variant antigens formed by the recombination and exchange of genes encoding glycophorin A (GPA) and glycophorin B (GPB). It consists of Mia, Vw, Eleven antigens, including Mur, Hil, Hut, MUT, Hop, Nob, DANE, TSEN and MINY, formed eleven different phenotypes. Among them, Mia and Mur, which often cause acute hemolytic transfusion reactions and hemolytic disease of the newborn, have received more and more clinical attention. [0003] Mia antigen (...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2531/113C12Q2545/101
Inventor 魏双施李勇蒙青林王红梅段生宝田晶晶丁少华陈晔洲李冬
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI