Method of treating hexavalent chromium wastewater by immobilized microorganisms

A technology of immobilized microorganisms and hexavalent chromium, which is applied in the field of immobilized microorganisms to treat hexavalent chromium wastewater, can solve the problems of hexavalent chromium wastewater polluting the environment and affecting human health, and achieves strong poison resistance, good impact resistance, Simple effect of running equipment

Inactive Publication Date: 2015-03-04
SOUTHWEAT UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for treating hexavalent chromium wastewater with immobilized microorganisms in view of the serious pollution of the environment by hexavalent chromium wastewater and the impact on human health.

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  • Method of treating hexavalent chromium wastewater by immobilized microorganisms
  • Method of treating hexavalent chromium wastewater by immobilized microorganisms
  • Method of treating hexavalent chromium wastewater by immobilized microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Tested strain XJ-Ⅱ: the dominant strain isolated from saline-alkali land in Xinjiang, identified as Bacillus subtilis (Bacillus subtilis), the optimum growth temperature is 37°C.

[0049] This example is used to determine the effect of XJ-II on removing hexavalent chromium ions, and the specific operation is as follows.

[0050] Take the slant strain XJ-Ⅱ, inoculate it in 100mL liquid medium, and culture it in a constant temperature shaking incubator at 37°C and 120r / min for 18h to obtain a seed solution. Take OD 600 =0.8 seed solution, inoculated in Cr(Ⅵ) solutions with different concentrations with 10% inoculum amount. Take out a certain amount of liquid at regular intervals within 60 hours, and measure the concentration OD of the bacterial solution 600 , each experiment was repeated three times, the same below, and the culture medium was used as the control. The supernatant was collected by centrifugation, and the residual Cr(VI) content in the solution was measur...

Embodiment 2

[0059] The influence of embodiment 2 additives on immobilized microorganism treatment Cr(Ⅵ)

[0060] 2.1 Experimental method

[0061] CNTs (carbon nanotubes), activated carbon and straw were used as additives to study the immobilization technology, and Cr(Ⅵ) solution was processed.

[0062] Take 100mL and incubate for 14h, OD 600 = 2.0 bacteria (XJ-II) precipitated after centrifugation, dissolved in 10mL SA solution with a final concentration of 1.5%, and then added 0.05g, 0.1g, 0.15g, 0.2g and 0.25g of a single additive, and stirred evenly Drop into 1% calcium chloride solution, take out after immobilization for 1 hour, wash with normal saline, and set aside.

[0063] The prepared immobilized microbial particles containing additives were added to 100mL of 10mg / L Cr(VI) solution, and the residual Cr(VI) content in the solution was measured at intervals within 60h. The immobilized granules without additives were used as control 1, the immobilized granules containing 0.25 g o...

Embodiment 3

[0089] The composition of the LB medium used in this example is as follows: yeast powder: 5g; peptone: 10g; NaCl: 10g; agar: 15g; distilled water: 1000mL. Adjust pH 7.2-7.4 with 10% HCl / NaCl. When preparing liquid medium, there is no need to add agar.

[0090] In this example, the preparation method of immobilized microbial particles is as follows: Take 100mL of culture for 14h, OD 600 =2.0 bacteria (XJ-Ⅱ) precipitated after centrifugation, dissolved in 10mL SA solution with a final concentration of 1.5%, then added 0.25g of CNTs, stirred evenly, dropped into 1% calcium chloride solution, immobilized for 1h Then take it out, wash it with normal saline, and set it aside.

[0091] Add 20%, 40%, 60%, 80%, and 100% LB medium to 10 mg / L Cr(Ⅵ) solution, treat 100 mL of the above solution with immobilized microbial particles, and investigate the effect of the amount of medium on the immobilized microorganism. Effect of particle treatment on Cr(VI). Wherein, in the 10mg / L Cr(Ⅵ) so...

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Abstract

The invention discloses a method of treating hexavalent chromium wastewater by immobilized microorganisms. The method aims at solving the problem that the human health is affected as the environment is severely polluted by the hexavalent chromium wastewater. The method comprises the following steps: a step I of preparing immobilized microorganism particles and a step II of treating wastewater, wherein in the step I, a microbial agent is dissolved into a sodium alginate solution, an additive is added to form a mixed solution, then the mixed solution is added into a calcium chloride solution to obtain embedded particles, and the embedded particles are stewed and immobilized, and washed by normal saline to obtain the immobilized microorganism particles. According to the method of treating hexavalent chromium wastewater by immobilized microorganisms disclosed by the invention, by selecting and matching the additive and a culture medium, the treatment effects and treatment efficiency of the hexavalent chromium wastewater can be greatly improved to meet the needs of industrially treating the hexavalent chromium wastewater. The method of treating hexavalent chromium wastewater by immobilized microorganisms has the advantages of low cost, small equipment investment, convenience in operation, and the like, has a wide market prospect and a high application value, and is worthy of being popularized and applied on a large scale.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to the treatment of wastewater containing hexavalent chromium, in particular to a method for treating hexavalent chromium wastewater with immobilized microorganisms. Background technique [0002] As an important metal element, chromium is one of the most widely used metals in industry. Due to the extensive application of chromium, a large amount of chromium-containing wastewater is produced, and industries such as electroplating, metallurgy, leather, paint, pigment, printing and dyeing, pharmaceuticals and the manufacture of special steel are the main sources of Cr(Ⅵ)-containing wastewater. In industrial chromium-containing wastewater, chromium mainly exists in the hexavalent form (Cr(Ⅵ)). The toxicity of chromium is related to the valence state of its existence. Hexavalent chromium is 100 times more toxic than trivalent chromium; because chromium has "three-induced" effects, and it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N11/10C12N11/04
CPCC02F3/348C02F2101/22C12N1/20C12N11/04C12N11/10
Inventor 陈晓明许燕王丹唐运来罗学刚伍迪陈彩霞
Owner SOUTHWEAT UNIV OF SCI & TECH
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