Production method of earthworm blood cell smear

A production method and technology of blood cells, which are applied in the field of soil pollution ecotoxicological diagnosis smears, can solve the problems that cannot meet the needs of soil ecological toxicological diagnosis, and achieve the effect of good staining effect and easy operation

Inactive Publication Date: 2015-03-04
SHENYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the in-depth research on ecotoxicological diagnosis of soil pollution, these indicators can no longer meet the needs of soil ecological toxicological diagnosis under low-dose pollution conditions, and the research on earthworm genetic toxicology has gradually become a research hotspot of pollution indicators.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Taking blood from earthworms: wash the live Eisenia earthworms with annulus with distilled water, put them in a petri dish and add absolute ethanol drop by drop for anesthesia, after 3 minutes, touch the earthworms with tweezers, and wait until they stop After the reaction, take it out, and use filter paper to absorb the water on the surface of the earthworm, and then use surgical scissors to cut the abdominal blood vessel below the earthworm annulus and near the abdomen of the annulus, so that the blood flows out naturally, do not squeeze, take a clean glass slide, Let the blood drop 4 mm away from one end of the glass slide, and do not touch the surface of the earthworm.

[0017] 2. Smear: Take a glass slide with smooth edges as a slide, place it in front of the blood drop, move it backwards and touch the blood drop, and when the blood is evenly attached between the two slides, make the two slides The film is at an angle of about 30 degrees, and it is pushed out st...

Embodiment 2

[0021] 1. Taking blood from earthworms: wash the live Eisenia earthworms with annulus with distilled water, put them in a petri dish and add absolute ethanol drop by drop for anesthesia, after 3 minutes, touch the earthworms with tweezers, and wait until they stop After the reaction, take it out, and use filter paper to absorb the water on the surface of the earthworm, and then use surgical scissors to cut the abdominal blood vessel below the earthworm annulus and near the abdomen of the annulus, so that the blood flows out naturally, do not squeeze, take a clean glass slide, Let the blood drop 5 mm away from one end of the glass slide, and do not touch the surface of the earthworm.

[0022] 2. Smear: Take a glass slide with smooth edges as a slide, place it in front of the blood drop, move it backwards and touch the blood drop, and when the blood is evenly attached between the two slides, make the two slides The film is at an angle of about 35 degrees, and is pushed out stead...

Embodiment 3

[0026] 1. Taking blood from earthworms: wash the live Eisenia earthworms with annulus with distilled water, put them in a petri dish and add absolute ethanol drop by drop for anesthesia, after 3 minutes, touch the earthworms with tweezers, and wait until they stop After the reaction, take it out, and use filter paper to absorb the water on the surface of the earthworm, and then use surgical scissors to cut the abdominal blood vessel below the earthworm annulus and near the abdomen of the annulus, so that the blood flows out naturally, do not squeeze, take a clean glass slide, Let the blood drop 4 mm away from one end of the glass slide, and do not touch the surface of the earthworm.

[0027] 2. Smear: Take a glass slide with smooth edges as a slide, place it in front of the blood drop, move it backwards and touch the blood drop, and when the blood is evenly attached between the two slides, make the two slides The film is at an angle of 40 degrees, and it is pushed out smoothly...

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PUM

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Abstract

The invention provides a production method of an earthworm blood cell smear, and relates to a production method of a soil pollution ecotoxicology diagnosis smear. The method comprises the following steps: obtaining blood from earthworms, smearing, dyeing and observing; and specially comprises the steps: washing living earthworms with clitellum by distilled water; putting the washed earthworms into a culture dish and gradually dripping absolute ethyl alcohol in the culture dish for carrying out anesthesia; then shearing abdomen blood vessels at the abdomen, below the earthworm clitellum and close to the clitellum by surgical scissors; dripping the blood on a glass slide; putting another glass slide in front of a blood drop and moving the another glass slide backwards to be in contact with the blood drop; after the blood is uniformly attached between the two glass slides, naturally airing; dripping Giemsa dyeing liquid on a blood film and washing off the dyeing liquid by the distilled water; absorbing surplus water with filter paper; and after naturally airing, clearly observing forms of all cells in earthworm blood cells. The invention provides a method and technology support for soil pollution ecotoxicology diagnosis.

Description

technical field [0001] The invention relates to a method for making a smear for soil pollution ecotoxicology diagnosis, in particular to a method for making an earthworm blood cell smear. Background technique [0002] Earthworms are one of the most important habitats in soil ecosystems. In the process of soil formation, earthworms play a unique role in the maintenance of soil structure and soil fertility. Earthworms can improve soil aeration, water retention and drainage, increase soil nutrient cycle speed, adjust the composition of soil microbial flora, improve functions of soil ecosystems. In addition, earthworms have a special status in the terrestrial ecosystem. Many animals, mice, birds, etc. can eat earthworms. In a sense, it connects the pedosphere and the atmosphere, and communicates the connection between soil organisms and above-ground organisms. [0003] In the study of soil pollution ecotoxicology diagnosis, because earthworms are widely distributed and are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 宋雪英梁茹晶胡晓钧胡爽李玉双侯永侠杨继松
Owner SHENYANG UNIV
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