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MAP2K1 marker for diagnosing closing or opening of arterial ducts

A technology of patent ductus arteriosus and reagents, which is applied in the field of clinical diagnosis of molecular biology and medicine, and can solve problems that are generally completed within 10 to 15 hours after birth.

Inactive Publication Date: 2015-03-11
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually completed within 10 to 15 hours after birth, but has the potential to reopen within 7 to 8 days
There is no report on the diagnostic markers of ductus arteriosus closure

Method used

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  • MAP2K1 marker for diagnosing closing or opening of arterial ducts
  • MAP2K1 marker for diagnosing closing or opening of arterial ducts
  • MAP2K1 marker for diagnosing closing or opening of arterial ducts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Materials and methods

[0024] 1.1 Isolation of human ductus arteriosus tissue

[0025] All steps and processes of this experiment were approved by the Ethics Committee of Shanghai Jiao Tong University School of Medicine. Open (n=10, age=30.2±6.3 days) or closed (n=10, age=25.3±7.9 days) ductus arteriosus tissues were obtained from children with ductus arteriosus-dependent congenital heart disease during surgery (patients with See Table 1 for details. All patients received prostaglandin E1 before surgery, but the ductus arteriosus in the closed group remained closed before surgery. The status of the ductus arteriosus (patent or closed) was confirmed by echocardiography and CT and confirmed during the procedure. All samples were washed with saline, divided into four parts, and quickly frozen in liquid nitrogen.

[0026] Table 1 Clinical information of children

[0027]

[0028] Note: *P<0.05 open group vs closed group; TGA / IVS: complete transposition of the gr...

Embodiment 2

[0060] 20 patients with suspected patent ductus arteriosus were diagnosed using the relative content of MAP2K1 protein as a marker. Adopt Western blotting to analyze, and specific method is the same as embodiment 1. The relative expression level of MAP2K1 = 3.00 (MAP2K1 / GAPDH) was used as the diagnostic cut-off value, which was judged as patent ductus arteriosus above the above cut-off value. The diagnostic results of MAP2K1 protein markers showed that 14 patients had patent ductus arteriosus. In the later stage, all 14 patients underwent ductus arteriosus closure surgery, and all 14 patients were confirmed to have patent ductus arteriosus during the operation. Five of the other six patients underwent further cardiac surgery, and it was confirmed that none of the five had a patent ductus arteriosus.

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Abstract

The invention relates to an MAP2K1 marker for diagnosing the closing or opening of arterial ducts. The opened and closed arterial duct tissues are identified and analyzed through an ITRAQ proteomics method, and 132 different proteins are screened out; after signal path analysis is carried out, an MAP2K1 protein is selected; the MAP2K1 protein is identified with three methods including western blot, Qrt-PCR and immunofluorescence analysis, the expression of the protein in the arterial duct tissues of a human body is proved for the first time, and the protein has a significant difference in the closed and opened arterial duct tissues, so that the protein can be a key factor for regulating and controlling the closing or opening of the arterial ducts. The MAP2K1 protein can be used for clinical diagnosis of patent ductus arteriosus, and the accuracy of diagnosis is high.

Description

technical field [0001] The present invention relates to the technical field of clinical diagnosis of molecular biology and medicine, in particular, relates to MAP2K1 as a marker for diagnosing closed or open ductus arteriosus. Background technique [0002] The ductus arteriosus is a conduit between the aorta and the pulmonary artery, an important pathway for fetal circulation. After the birth of a child, the ductus arteriosus is functionally closed, and the vast majority of babies gradually close anatomically to become a ligament arteriosus about 3 months after birth. If it is not closed, it is called a patent ductus arteriosus. The closure of the ductus arteriosus is divided into two phases: 1. The first phase is the physiological closure phase. After the baby is born and cries, the first breath inhales, the alveoli will expand, and the pulmonary vascular resistance will decrease accordingly. The blood flow of the pulmonary artery will directly enter the lungs to establish...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6803G01N2800/329
Inventor 洪海筏刘锦纷叶霖财
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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