Method for identifying quality of steamed pork skin by observing microstructure
A technology of microstructure and skinning, which is applied in the direction of material analysis by measuring secondary emissions, to achieve a high degree of standardization, intuitive and accurate identification results, and good repeatability
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Example Embodiment
[0015] Example 1
[0016] experiment procedure:
[0017] 1) Take the pork skins obtained from the frying temperature of 100℃, 160℃, 180℃, 200℃, 220℃, 230℃ in the same time and name them A 1 , A 2 , A 3 , A 4 , A 5 , A 6 ;
[0018] 2) Cut the meat skin into thin slices of 3×15mm, immerse it in 2.6% glutaraldehyde solution, and fix it at 5℃ for 23h;
[0019] 3) Pour out the fixative solution, and rinse the sample 4 times with 0.1M, pH 7.5 phosphate buffer for 3 minutes each time;
[0020] 4) Dehydrate the samples with ethanol solutions (ethanol: water, v / v) with gradient concentrations of 15%, 35%, 55%, 75% and 90% respectively. Each concentration is dehydrated for 22 minutes, and then anhydrous Ethanol dehydration treatment twice, 22min each time. Finally, the sample was treated with a mixture of absolute ethanol and isoamyl acetate at a ratio of 1:1 (anhydrous ethanol: isoamyl acetate, v / v) for 32 minutes, and then treated with pure isoamyl acetate for 12 hours;
[0021] 5) The obtaine...
Example Embodiment
[0026] Example 2
[0027] experiment procedure:
[0028] 1) Take the minced pork skin obtained at the same frying temperature (230℃) and the frying time of 0min, 5min, 8min, 11min, 14min, 17min, and 20min, respectively named as B 1 , B 2 , B 3 , B 4 , B 5 , B 6 , B 7 ;
[0029] 2) Cut the meat skin into thin slices of 3×15mm, immerse it in 2.6% glutaraldehyde solution, and fix it at 5℃ for 23h;
[0030] 3) Pour out the fixative solution, and rinse the sample 4 times with 0.1M, pH 7.5 phosphate buffer for 3 minutes each time;
[0031] 4) Dehydrate the samples with ethanol solutions (ethanol: water, v / v) with gradient concentrations of 15%, 35%, 55%, 75% and 90% respectively. Each concentration is dehydrated for 22 minutes, and then anhydrous Ethanol dehydration treatment twice, 22min each time. Finally, the sample was treated with a mixture of absolute ethanol and isoamyl acetate at a ratio of 1:1 (anhydrous ethanol: isoamyl acetate, v / v) for 32 minutes, and then treated with pure isoa...
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