Method for identifying quality of steamed pork skin by observing microstructure
A technology of microstructure and skinning, which is applied in the direction of material analysis by measuring secondary emissions, to achieve a high degree of standardization, intuitive and accurate identification results, and good repeatability
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Embodiment 1
[0016] experiment procedure:
[0017] 1) Take pork skins obtained by frying at 100°C, 160°C, 180°C, 200°C, 220°C, and 230°C within the same time period, and name them as A 1 、A 2 、A 3 、A 4 、A 5 、A 6 ;
[0018] 2) Flatten and longitudinally cut the pork skin into thin slices of 3×15 mm, completely immerse it in 2.6% glutaraldehyde solution, and fix it at 5°C for 23 hours;
[0019] 3) Pour off the fixative, and rinse the sample with 0.1M, pH7.5 phosphate buffer solution continuously for 4 times, each time for 3 minutes;
[0020] 4) The samples were dehydrated with ethanol solutions (ethanol: water, v / v) with gradient concentrations of 15%, 35%, 55%, 75% and 90%, respectively, each concentration was dehydrated for 22 minutes, and then dehydrated with anhydrous Ethanol dehydration treatment twice, each 22min. Finally, treat the sample with absolute ethanol and isoamyl acetate at a ratio of 1:1 (absolute ethanol: isoamyl acetate, v / v) to treat the sample for 32 minutes, and...
Embodiment 2
[0027] experiment procedure:
[0028] 1) Under the same frying temperature (230°C) and the frying time is 0min, 5min, 8min, 11min, 14min, 17min, 20min, the pork skins are named as B 1 , B 2 , B 3 , B 4 , B 5 , B 6 , B 7 ;
[0029] 2) Flatten and longitudinally cut the pork skin into thin slices of 3×15 mm, completely immerse it in 2.6% glutaraldehyde solution, and fix it at 5°C for 23 hours;
[0030] 3) Pour off the fixative, and rinse the sample with 0.1M, pH7.5 phosphate buffer solution continuously for 4 times, each time for 3 minutes;
[0031] 4) The samples were dehydrated with ethanol solutions (ethanol: water, v / v) with gradient concentrations of 15%, 35%, 55%, 75% and 90%, respectively, each concentration was dehydrated for 22 minutes, and then dehydrated with anhydrous Ethanol dehydration treatment twice, each 22min. Finally, treat the sample with absolute ethanol and isoamyl acetate at a ratio of 1:1 (absolute ethanol: isoamyl acetate, v / v) to treat the samp...
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