Method for extracting gynostemma pentaphyllum saponins from gynostemma pentaphyllum

A technology of Gypenoside and Gynostemma pentaphyllum, which is applied in the extraction field of biotechnology, can solve the problems of long production cycle, safety impact, sample dead adsorption, etc., and achieve the effect of increasing process time and cost, improving production efficiency, and increasing dissolution rate

Inactive Publication Date: 2015-03-25
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of Gypenoside include water extraction, organic solvent extraction, microwave-assisted extraction, ultrasonic-assisted extraction, enzymatic extraction and other methods. The above methods generally use macroporous resin purification as the main separation step after extraction. However, with the macroporous resin purification method, the resin needs to be treated before use, and the purification needs to go through the process of adsorption and elution, and the production cycle is long
When using a macroporous resin for purification and elution, due to physical a

Method used

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  • Method for extracting gynostemma pentaphyllum saponins from gynostemma pentaphyllum

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Effect test

Embodiment 1

[0025] A method for extracting gypenoside from Gynostemma pentaphyllum, specifically comprising the steps of:

[0026] 1) Raw material processing: breaking fresh Gynostemma pentaphyllum into pieces;

[0027] 2) High-pressure extraction: Put the processed raw materials into a high-pressure tank, add the same weight of pure water, add polyether modified silicone equivalent to 0.25% of the raw material weight, and pressurize to 4kgf / cm 2 , to make the pressure uniform, and keep it for 10 minutes, return to normal pressure, filter, and obtain the extract;

[0028] 3) Separation: Gynostemma pentaphyllum is carried out two-phase extraction to obtain the upper phase of extraction and the lower phase of extraction. The two-phase system used in two-phase extraction is composed of polyethylene glycol, sodium hydroxide and water. Polyethylene glycol is in The mass percent in the two-phase aqueous system is 20%, the mass percent of sodium hydroxide in the two-phase aqueous system is 10%,...

Embodiment 2

[0032] A method for extracting gypenoside from Gynostemma pentaphyllum, specifically comprising the steps of:

[0033] 1) Raw material processing: pulverizing the dried Gynostemma pentaphyllum;

[0034] 2) High-pressure extraction: Put the treated raw materials into a high-pressure tank, add the same weight of purified water, add polyether modified silicone equivalent to 0.05% of the weight of raw materials, and pressurize to 4kgf / cm 2 , make the pressure uniform, and keep it for 15 minutes, return to normal pressure, filter, and obtain the extract;

[0035] 3) Separation: Gynostemma pentaphyllum is carried out two-phase extraction to obtain the upper phase of extraction and the lower phase of extraction. The two-phase system used in two-phase extraction is composed of polyethylene glycol, sodium hydroxide and water. Polyethylene glycol is in The mass percent in the two-phase aqueous system is 15%, the mass percent of sodium hydroxide in the two-phase aqueous system is 15%, t...

Embodiment 3

[0039] A method for extracting gypenoside from Gynostemma pentaphyllum, specifically comprising the steps of:

[0040] 1) Raw material processing: pulverizing the dried Gynostemma pentaphyllum;

[0041] 2) High-pressure extraction: Put the processed raw materials into a high-pressure tank, add the same weight of purified water, add polyether modified silicone equivalent to 0.15% of the raw material weight, and pressurize to 4kgf / cm 2 , to make the pressure uniform, and keep it for 12 minutes, return to normal pressure, and filter to obtain the extract;

[0042] 3) Separation: Gynostemma pentaphyllum is carried out two-phase extraction to obtain the upper phase of extraction and the lower phase of extraction. The two-phase system used in two-phase extraction is composed of polyethylene glycol, sodium hydroxide and water. Polyethylene glycol is in The mass percent in the two-phase aqueous system is 25%, the mass percent of sodium hydroxide in the two-phase aqueous system is 12%...

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Abstract

The invention discloses a method for extracting gynostemma pentaphyllum saponins from gynostemma pentaphyllum. The method comprises the following steps of treating raw materials, carrying out high-pressure extraction, carrying out aqueous two-phase extraction, desalting, concentrating and drying, thereby obtaining the gynostemma pentaphyllum saponins. The method has the advantages of high content, high extraction ratio, simple process, suitability in production, low production cost and the like.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting gypenoside from gynostemma pentaphyllum. Background technique [0002] Gynostemma, a perennial herbaceous vine, also known as paradise grass, gospel grass, super ginseng, compass bottom, rooting everywhere, aescin, five-leaf ginseng and seven-leaf ginseng, etc. It is called Ganman tea in Japan and is a perennial of the genus Gynostemma cucurbitaceae. Herbaceous vines. Gynostemma likes a humid and mild climate, and it mostly grows wild in shaded places such as forests and streams, and is a perennial climbing herb. In China, it is mainly distributed in Shaanxi Pingli, Gansu Kangxian, Hunan, Hubei, Yunnan, Guangxi and other provinces. It is known as "Southern Ginseng". "In 1986, the National Science and Technology Commission listed Gynostemma Gynostemma as the top of the "precious Chinese medicinal materials" to be developed in the "Spar...

Claims

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Application Information

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IPC IPC(8): A61K36/424
CPCA61K36/424A61K2236/15A61K2236/333A61K2236/37A61K2236/51A61K2236/53A61K2236/55
Inventor 卢照凯钟德品谢冬养周晓青卢桦香应启实陆美珍
Owner 桂林三宝生物科技有限公司
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