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A method for crushing oil-producing microorganism cells by pressurized and heated jet

An oil-producing microorganism and cell crushing technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of chemical reagents that are not friendly to the environment, not suitable for large-scale application, and have a large environmental impact, and achieve saving Processing time, processing time saving, environment-friendly effect

Inactive Publication Date: 2017-04-05
HENAN TIANGUAN GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent application number 200910188094.9 discloses a method for rapidly breaking microalgae cells. After the broken microalgae is mixed with liquid nitrogen, it is mechanically ground and crushed to obtain broken cell microalgae. After being illuminated, it is used for oil extraction. This method is costly and has a large impact on the environment, so it is not suitable for large-scale application
Patent application number 201110435889.2 provides a microalgae treatment method. This method requires pretreatment of the material such as pH adjustment, and then material impact. The added chemical reagent is not friendly to the environment, and it is only suitable for water content up to 85%. The above microalgae solution

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  • A method for crushing oil-producing microorganism cells by pressurized and heated jet

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 1L of oleaginous heterotrophic chlorella with a water content of 95%, apply pressure to the material by a high-pressure pump, and spray it out through the delivery pipeline at a speed of 6 times the speed of sound, while the temperature of the pipeline rises to 100 degrees Celsius, and then the sprayed The chlorella material hits the steel plate at an angle of 110 degrees. The high-speed moving chlorella rebounds after encountering obstacles and hits another steel plate again. The impact is continuous for 3 times, and the material is discharged, and then the broken pellets are Oil extraction from algae material.

[0034] According to experiments, the cell crushing rate of Chlorella is 100%, and the oil extraction rate is 98.6%.

Embodiment 2

[0036] Take 1L of oleaginous heterotrophic chlorella with a water content of 95%, apply pressure to the material by a high-pressure pump, and spray it out through the delivery pipeline at a speed of 7 times the speed of sound, while the temperature of the pipeline rises to 130 degrees Celsius, and then the sprayed The chlorella material hits the steel plate at an angle of 140 degrees. The high-speed moving chlorella rebounds after encountering obstacles and hits another steel plate again. The impact is continuous for 5 times, and the material is discharged, and then the broken pellets are Oil extraction from algae material.

[0037] According to experiments, the cell crushing rate of chlorella is 100%, and the oil extraction rate is 99.7%.

Embodiment 3

[0039] Take 1L of oil-producing heterotrophic Chlorella with a water content of 95%, apply pressure to the material by a high-pressure pump, and spray it out through the delivery pipeline at a speed of 8 times the speed of sound, while the temperature of the pipeline rises to 160 degrees Celsius, and then the sprayed The chlorella material hits the steel plate at an angle of 120 degrees, and the high-speed moving chlorella rebounds after encountering obstacles, and hits another steel plate again, hitting 6 times in a row, discharging, and then crushing the broken pellets Oil extraction from algae material.

[0040] According to experiments, the cell crushing rate of chlorella is 100%, and the oil extraction rate is 99.6%.

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Abstract

The invention provides a method for crushing oil-producing microorganism cells by pressurized and heated jets, and belongs to the technical field of cell crushing. A method for crushing oil-producing microbial cells by pressurized and heated jets. After the oil-producing microbial cells are pressurized, they are sprayed out through a pipeline at a speed of 6 to 10 times the sound speed, and the temperature of the pipeline is raised to 100-200 degrees Celsius at the same time, and then the spraying The oil-producing microbial cells hit the inner walls of two parallel and opposite hard plates at an angle of 15-165 degrees, and repeatedly hit 3-10 times. The invention can realize the crushing of oil-producing microbial cells in a one-time and high-efficiency manner, and the operation process is simple, without pretreatment of raw materials, without adding any chemical or biochemical reagents, the whole crushing process is environmentally friendly, pollution-free, and the crushing rate is 100% %, can be widely used in the technical field of crushing oil-producing microbial cells.

Description

technical field [0001] The invention belongs to the technical field of cell crushing, and in particular relates to a cell crushing method of oil-producing microorganisms by pressurized and heated jets. Background technique [0002] Currently, cell disruption methods mainly include physical disruption, chemical and biochemical methods. [0003] Physical crushing methods include high-pressure homogenization, bead milling, freeze-thaw crushing, microwave, ultrasonic crushing and other methods, which are characterized by large processing capacity, high crushing efficiency, and fast speed. During the processing, the cell wall cannot withstand strong mechanical shearing force and fragmentation, resulting in cytoplasmic efflux. The mechanical crushing method mainly relies on high power to achieve mechanical shear force, and for oil-producing microorganisms with thick cell walls, it usually cannot achieve the expected crushing effect at one time or in a short period of time, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/06C12R1/89C12R1/645
CPCC12N1/066
Inventor 毕生雷杜风光刘钺乔建援刘晓菊郑世文金洪波郑彬薛宝丁凌飞张喆王凌如
Owner HENAN TIANGUAN GRP