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Compound extracted and separated from schisandra chinensis as well as preparation method and application of compound

A compound, the technology of Schisandra chinensis, applied in the field of phytochemistry, can solve the problems of undiscovered drugs and other problems, and achieve the effect of easy extraction and separation, good reference value and high yield

Active Publication Date: 2015-04-01
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] And the inventor finds that when the active ingredient of Schisandra Fructus is researched, the sesquiterpene compound contained in Schisandra Fructus also has the effect of suppressing tumor cells, has not yet seen the report of relevant sesquiterpene compound to suppressing tumor cell simultaneously at present, more No drugs related to this compound have been found on the market

Method used

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  • Compound extracted and separated from schisandra chinensis as well as preparation method and application of compound
  • Compound extracted and separated from schisandra chinensis as well as preparation method and application of compound
  • Compound extracted and separated from schisandra chinensis as well as preparation method and application of compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Extraction and separation of compounds

[0032] Take 500g of dried fruit of Schisandra chinensis, add 6 times of ethanol with a weight concentration of 90% after crushing, heat and reflux for extraction 5 times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and obtain 300ml of concentrated extracts, and after standing for 24 hours, the upper layer is obtained. Oil layer 120g; The obtained oil layer was dispersed with 120g ethyl acetate, and the sample was mixed with 2 times of the quality of the oil layer, and the obtained silica gel mixed sample was subjected to normal phase silica gel column chromatography, and 10 times of silica gel was used as a filler, and the column was wet-packed. For sample loading by dry method, use petroleum ether:ethyl acetate volume ratio 10:1 as the eluent for atmospheric pressure elution, collect the eluent in separate tubes, each tube is 100ml, and use n-hexane:acetone=10 for each: 1.5...

Embodiment 2

[0033] Example 2 Extraction and separation of compounds

[0034]Take 500g of dried fruit of Schisandra chinensis, add 10 times of ethanol with a weight concentration of 95% to heat and reflux for extraction 4 times after crushing, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and obtain 400ml of concentrated extracts, and after standing for 24 hours, the upper layer is obtained. Oil layer 130g; Gained oil layer is dispersed with 130g ethyl acetate, mixes sample with 2.5 times of the quality of oil layer with silica gel, and the obtained silica gel mixed sample is carried out normal phase silica gel column chromatography, with 15 times of silica gel as filler, wet packing column, For sample loading by dry method, use petroleum ether:ethyl acetate volume ratio 10:1 as the eluent for atmospheric pressure elution, collect the eluent in separate tubes, each tube is 100ml, and use n-hexane:acetone=10 for each: 1.5 is the developer for thin...

Embodiment 3

[0035] Example 3 Extraction and separation of compounds

[0036] Take 500g of dried fruit of Schisandra chinensis, add 12 times of ethanol with a weight concentration of 92% to extract 3 times under reflux after crushing, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and obtain 500ml of concentrated extracts, and after standing for 24 hours, the upper layer is obtained. Oil layer 150g; The obtained oil layer was dispersed with 150g ethyl acetate, and the sample was mixed with 3 times of the quality of the oil layer, and the obtained silica gel mixed sample was subjected to normal phase silica gel column chromatography, and 20 times of silica gel was used as a filler, and the column was wet-packed. For sample loading by dry method, use petroleum ether:ethyl acetate volume ratio 10:1 as the eluent for atmospheric pressure elution, collect the eluent in separate tubes, each tube is 100ml, and use n-hexane:acetone=10 for each: 1.5 is the...

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Abstract

The invention provides a compound extracted and separated from schisandra chinensis as well as a preparation method and application of the compound, and belongs to the technical field of plant chemistry. The compound has structural characteristics described in the descriptions, and has a chemical name of 3,7,11,11-tetramethyl-1,5,6,7,9,10-hexahydrocyclopenta[c]pentalen-2-one. The preparation method comprises the following steps: grinding the schisandra chinensis, performing heating reflux extraction by ethanol, performing reduced pressure concentration until no ethanol smell is generated, standing for 24 hours, and siphoning upper-layer oil; dispersing by ethyl acetate, performing normal-phase silica-gel column chromatography and normal-pressure elution, collecting elution liquid, combining collected liquid containing target compounds according to a thin layer chromatography result, performing reduced pressure concentration at 45 DEG C to obtain extractum, dispersing the extractum by methanol, filtering by a filter membrane, performing high-pressure preparation and separation on filter liquor by C18 reversed phase chromatographic packing, collecting a corresponding chromatographic peak, and concentrating the collected liquid to dryness to obtain a light yellow oily product. The compound can be used for preparing drugs for inhibiting tumor cells.

Description

technical field [0001] The invention relates to a medicinal compound and its extraction method and use, in particular to a pharmacologically active compound extracted and separated from Schisandra chinensis, its method and use, and belongs to the technical field of phytochemistry. Background technique [0002] Schisandra chinensis Baill is the dry and mature fruit of Schisandra chinensis Baill. It is picked in autumn when the fruit is ripe and dried for medicinal purposes. Its nature is sour, sweet and warm. Antidiarrheal, promoting body fluid and quenching thirst, is generally used to treat deficiency cough and asthma, nocturnal emission and enuresis, kidney diarrhea and hyperhidrosis and thirst. It is usually decocted with other traditional Chinese medicines or made into Chinese patent medicines. However, with the development of science and technology and the deepening of comprehensive research on traditional Chinese medicine, traditional Chinese medicine has new developme...

Claims

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Application Information

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IPC IPC(8): C07C49/643C07C45/78A61P35/00
CPCC07C45/78C07C45/79C07C49/643C07C2603/10
Inventor 郑皓元李启发夏柯文焕松刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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