Immobilized culture method and device for microalgae
A technology of immobilized culture and microalgae, which is applied in the field of microalgae cultivation, can solve the problems of microalgae cell loss of activity, yield reduction, death, etc., and achieve the effect of avoiding loss of microalgae activity and increasing production
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Embodiment 1
[0092] This embodiment adopts the culture mode substantially identical with comparative example, and difference is:
[0093] The culture carrier cellulose acetate filter membrane is divided into upper and lower parts, so that the whole culture surface 20 forms two upper and lower subculture surfaces 21 and 22 respectively. In this way, if image 3 As shown, after culturing for 3 days, the two culture carriers were removed respectively, exchanged positions up and down and then pasted on the coarse cloth again; when culturing to the 7th day, the entire culture surface 20 had obvious growth.
[0094] Harvest after 7 days of cultivation, and measure the biomass. The results showed that the average growth rate of each layer of culture surface was 3g m -2 d -1 , the cell growth rate per unit area of the overall culture system is 30g m -2 d -1 .
[0095] Compared with the comparative example, the growth rate of Example 1 was increased by 20%, indicating that during the cultivat...
Embodiment 2
[0097] Such as Figure 4 As shown, in Example 2, the microalgae are cultivated by using two pieces of cultivation surfaces. The breeding surface 30 includes two upper and lower sub-culture surfaces 31, 32, which can be exchanged and moved up and down regularly manually or automatically.
[0098] Specifically, two aluminum-plastic panels with a length×width of 0.5m×0.7m and a thickness of 0.005m are fixed on the frame 50, the frame 50 is as Figure 5 shown can be turned by folding, or as Figure 6 As shown, the positions of the two aluminum-plastic panels can be freely exchanged up and down by sliding.
[0099] Cover a layer of cotton cloth on the surface of each aluminum-plastic panel, and fix a cloth water pipe on the top of each aluminum-plastic panel. Move each aluminum-plastic plate to a suitable height in turn, spray a certain amount of Scenedesmus cell culture solution on the surface of the cotton cloth, and the biomass density is 15g / m 2 , thereby forming two sub-cu...
Embodiment 3
[0108] Such as Figure 7-9 As shown, three plastic plates with a length × width of 0.5m × 0.7m and a thickness of 0.005m are fixed on the designed frame 62 with a length × width of 1.5m × 0.7m. Slideways and three draw-in slots 64 are all equipped with, and the plastic plates are fixed by bolts 63, so that the three plastic plates slide up and down and are disassembled step by step.
[0109] The surface of each plastic plate is covered and fixed with a layer of cotton cloth. When inoculating for the first time, one volume of Scenedesmus algae mud is evenly smeared and inoculated on the cotton cloth surface of the lower plastic plate, and two volumes of Scenedesmus algae mud is inoculated on the cotton cloth surface of the middle plastic plate. The volume of Scenedesmus algae mud is inoculated on the surface of the cotton cloth of the upper plastic plate, wherein one volume < two volumes < three volumes, thereby forming three sub-culture surfaces 41, 42, 43, and these three sub...
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Abstract
Description
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