A method of improving liver function
A technology of liver function and liver lipid, applied in the field of treating poor liver function, treating poor liver function or improving liver function, can solve the problems of absence and loss of insulin response, etc.
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Embodiment 1
[0092] The influence of embodiment 1-methazolamide on the ALT level of patients with type 2 diabetes mellitus
[0093] The safety and efficacy of methazolamide (40 mg administered twice daily) as a potential treatment for type 2 diabetes were evaluated in a 24-week randomized, placebo-controlled, double-blind clinical trial. The primary efficacy endpoint of the clinical trial was after 24 weeks of treatment, when using methazolamide, compared with placebo, HbA 1c Decrease from baseline (ΔHbA 1c ). The primary safety measure was the effect of methazolamide on venous blood gas parameters, a measure of acidosis, compared with placebo.
[0094] The initial participants in the clinical trial were type 2 diabetic patients who had not experienced any antidiabetic treatment before entering the trial. The trial was expanded to include subjects who had been on metformin for at least 3 months prior to trial entry (MET) and had been on a stable metformin dose for at least 8 weeks. T...
Embodiment 2
[0107] The effect of embodiment 2-methazolamide on db / db mouse liver lipid
[0108] All reagents were purchased from Sigma-Aldrich (Australia). Dosing solutions of methazolamide acetazolamide in 65:35 (v / v) sterile saline:PEG400 were prepared fresh daily, protected from light and stored at room temperature. Male db / db mice (Animal Resource Centre, Australia) were maintained with free access to water and food (standard rodent diet: Barastoc Rat and Mouse, Ridley Agriproducts, Australia). Room temperature was maintained at 21 ± 2°C with a humidity of 40%-70% while a 12-h light / dark cycle was performed. Mice (n=4 per group) were treated daily with methazolamide (50 mg / kg / day) or vehicle by single-dose oral gavage for 9 days.
[0109]Blood samples were taken daily from the tip of the tail of each mouse, and glucose levels were measured with a glucometer (AccuCheck II; Roche, Australia). At the end of the study, animals were sacrificed humanely and a portion of liver tissue (l...
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