Implantable bioartificial liver

Abstract

The invention provides a combining reagent for preparing a bioartificial liver stent. The reagent comprises perfusate 1, perfusate 2, perfusate 3, perfusate 4, eluent 1 and eluent 2, wherein the perfusate is alkalescent hypotonic solution or pure water; the perfusate 2 is a group of ionic detergent aqueous solutions with gradient concentration; the perfusate 3 is aqueous electrolyte solution; theperfusate 4 is anticoagulant factor aqueous solution; the eluent 1 is distilled water; and the eluent 2 is non-ionic detergent aqueous solution. The invention also provides a method for preparing an implantable bioartificial liver by using the combining reagent, and the implantable bioartificial liver prepared by using the method. The bioartificial liver stent prepared by using the combining reagent is decellularized completely; and the structure of an extracellular matrix is kept complete. The bioartificial liver can accommodate a large number of cells and provide adequate blood supply, so that the survival time of a rate with an acute hepatic failure is prolonged obviously; and a liver function is improved obviously. Therefore, the bioartificial liver has an extremely good clinical application prospect.

Description

technical field [0001] The invention belongs to the fields of clinical medicine, biomedicine and regenerative medicine, relates to tissue engineering and artificial organ technology, in particular to an implanted bioartificial liver. Background technique [0002] As one of the most important organs of the human body, the liver undertakes multiple complex functions such as synthesis, secretion, metabolism, and detoxification. Once a large number of liver cells are necrotic due to various reasons, liver failure will occur, resulting in metabolic disorders and toxic substances. accumulation, affecting health. [0003] Orthotopic liver transplantation is the only effective method recognized so far for the treatment of end-stage liver failure caused by various reasons, but due to the severe shortage of donor livers, a large number of patients died while waiting for liver transplantation. Hepatocyte transplantation is attracting more and more attention due to its advantages of lo...

AI Technical Summary

Problems solved by technology

For example, in Ding Yitao et al., Research Progress of Bioartificial Liver in Treatment of Liver Failure, Journal of Internal Medicine, Volume 15, No. 3, 2009, it was disclosed that the current bioartificial liver scaffolds are almost all synthetic materials, but Synthetic material stents have two disadvantages that are difficult to overcome: on the one hand, because they are artificial synthetic materials, their biocompatibility is relatively low; Later, its blood supply is another problem

However, due to the exposure of collagen after treatment, severe blood coagulation will occur after implantation in the body, which will eventually lead to graft failure.

[0009] Due to the above defects, there are no reports of bioartificial livers prepared from decellularized natural artificial liver scaffolds that can be implanted in vivo.

[0010] At present, most of the transplantation sites for artificial livers are subcutaneous, spleen, pancreas, kidney capsule and intraperitoneal cavity, but they are all limited by the small amount of implanted liver cells and the inability to guarantee the blood supply, so that the implanted cells cannot eventually replace the organ function, leading to the failure of porting

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