Method suitable for tissue rapid cultivation of Acer rubrum

A red maple and tissue technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of not yet established the rapid propagation system of American red maple tissue, and achieve fine lateral roots, strong main roots and good seedling growth. Effect

Active Publication Date: 2015-04-15
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

So far, researchers have conducted preliminary research on the tissue culture technology of re

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  • Method suitable for tissue rapid cultivation of Acer rubrum
  • Method suitable for tissue rapid cultivation of Acer rubrum
  • Method suitable for tissue rapid cultivation of Acer rubrum

Examples

Experimental program
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[0025] Example 1: Primary culture

[0026] 1. The effect of different sterilization time combinations on the growth of explants

[0027] For the initial explants, select young shoots that were born in the same year, remove the leaves, cut the shoots into a single pair of bud stem segments with a length of 1 to 2 cm, soak them in a saturated washing powder aqueous solution for about 20 minutes, rinse them under running water for about 30 minutes, and transfer them to an ultra-clean workbench on. First use 75% alcohol to sterilize for 35, 50, 65 seconds, then use 0.1% mercury to sterilize for 8, 10, 12 minutes (1 to 2 times), then rinse with sterile water 4 times, 2min / time, and finally use The sterile filter paper absorbs the moisture on the surface of the explant. The specific time and frequency of each treatment are shown in Table 1. Inoculate on the culture medium under aseptic conditions, inoculate 10 explants, 1 explant / bottle for each treatment, repeat 3 times, after 10 days...

Example Embodiment

[0042] Example 2: Proliferation culture

[0043] After the axillary buds of the stem segment are elongated and have about 3 pairs of leaves, they are cut from the base, the leaves are removed and cut into 1-2cm long stems, and then transferred to the propagation medium. Using WPM as the basic medium and adding 0.65% agar powder, the sterile stem segments were respectively inoculated on the medium (pH=5.8) added with different concentrations of growth regulators and different concentrations of sugar for light culture. The concentration combinations of growth regulators are shown in Table 3, and the sugar concentration combinations are shown in Table 4. Each treatment is inoculated with 15 stem segments and 3 stem segments / bottle. Repeat 3 times. After 30 days of cultivation, observe the germination and growth of axillary buds. Calculate the proliferation coefficient, average plant height, and determine the optimal growth regulator and sugar addition amount for the proliferation me...

Example Embodiment

[0057] Example 3: Rooting culture

[0058] Select the sprouts that have been subcultured for more than 3 generations and the height is more than 2cm, cut off, and transferred to the rooting medium. Take WPM as the basic medium, add 0.65% agar powder and 0.1% activated carbon, and inoculate the sterile sprouts on the medium (pH=5.8) with different salt concentration, different growth regulator concentration, and different sugar concentration. Light cultivation. See Table 5 for each salt concentration combination, Table 6 for each growth regulator concentration combination, and Table 7 for each sugar concentration combination. Inoculate 10 stem segments and 2 stem segments / bottle for each treatment. Repeat 3 times. After 60 days of culture, Observe the root germination and growth, and calculate the rooting rate, average root number, average root length and average plant height to determine the best rooting medium.

[0059] Rooting rate (%)=number of roots / number of inoculation×100 ...

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Abstract

The invention discloses a method for tissue rapid cultivation of Acer rubrum. The method comprises the following steps: (1) conducting surface sterilization by adopting tender stem segments of Acer rubrum as explants; (2) cultivating on an initial medium for 25-30 days; (3) cultivating on a multiplying medium for 20-40 days; (4) conducting rooting cultivation on a rooting medium till generating appropriate roots. By adopting the method, excellent aseptic seedlings of Acer rubrum can be rapidly and constantly acquired, the survival rate of seedlings is high, technical guarantee is provided and solid foundation is established for industrialized seedling production of Acer rubrum, and industrialized development of Acer rubrum cultivation in China is facilitated.

Description

technical field [0001] The invention relates to a method suitable for rapid tissue propagation of American red maple. Background technique [0002] American red maple is a cultivar of Acer palmatum in the genus Aceraceae of the Aceraceae family. It is native to the east coast of the United States and has a wide range of adaptability. It can adapt to acidic soil, neutral soil and calcareous soil; Drought, humidity, and barren resistance, but likes a humid and warm climate, and grows well in a fertile soil and well-drained environment. American red maple is a large deciduous tree with 3-5 palmate leaves and 5-10cm in length. The young leaves are bright red or purple in spring, and turn slightly green in summer. The whole plant has a beautiful appearance, and the leaves often appear bright red in autumn. It is a well-known ornamental tree species for urban greening and landscape building, and is currently widely used in parks, courtyards, and community greening. The branches ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邱义兰刘如石廖旻晶
Owner HUNAN NORMAL UNIVERSITY
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