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Method suitable for tissue rapid cultivation of Acer rubrum

A red maple and tissue technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of not yet established the rapid propagation system of American red maple tissue, and achieve fine lateral roots, strong main roots and good seedling growth. Effect

Active Publication Date: 2015-04-15
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, researchers have conducted preliminary research on the tissue culture technology of red maple, but the tissue rapid propagation system of American red maple has not been established

Method used

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  • Method suitable for tissue rapid cultivation of Acer rubrum
  • Method suitable for tissue rapid cultivation of Acer rubrum
  • Method suitable for tissue rapid cultivation of Acer rubrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Primary culture

[0026] 1. The effect of different sterilization time combinations on the growth of explants

[0027] For the initial explants, select the young shoots of the year, remove the leaves, cut the shoots into 1-2cm long single-pair bud stem segments, soak them in saturated washing powder aqueous solution for about 20 minutes, rinse them under running water for about 30 minutes, and transfer them to the ultra-clean workbench superior. First use 75% alcohol to disinfect for 35, 50, and 65 seconds respectively, then use 0.1% mercury liter to disinfect for 8, 10, and 12 minutes respectively (disinfect in 1 to 2 times), then rinse with sterile water 4 times, 2 minutes each time, and finally use The surface moisture of the explants was blotted dry with sterile filter paper, and the specific time and frequency of disinfection for each treatment are shown in Table 1. Inoculate on the culture medium under sterile conditions, inoculate 10 explants for ...

Embodiment 2

[0042] Embodiment 2: proliferation culture

[0043] After the axillary buds of the stem segment elongate and have about 3 pairs of leaves, cut them off from the base, remove the leaves and cut them into 1-2 cm long stem segments and transfer them to the proliferation medium. Using WPM as the basic medium, adding 0.65% agar powder, inoculating the aseptic stem segments on the medium (pH=5.8) added with different concentrations of growth regulators and different concentrations of sugar for light culture. Each growth regulator concentration combination is shown in Table 3, and the sugar concentration combination is shown in Table 4. Each treatment was inoculated with 15 stem segments, 3 stem segments / bottle, and repeated 3 times. After cultivating for 30 days, the germination and growth of axillary buds were observed, and Calculate the proliferation coefficient and average plant height, and determine the optimal growth regulator and sugar addition in the proliferation medium.

...

Embodiment 3

[0057] Embodiment 3: rooting culture

[0058] Select the sprouts that have grown vigorously and reach a height of more than 2 cm after more than 3 generations of subculture, cut them off, and transfer them to the rooting medium. Take WPM as the basic medium, add 0.65% agar powder and 0.1% activated carbon, and inoculate the sterile sprouts on the medium (pH=5.8) with different salt concentrations, different growth regulator concentrations, and different sugar concentrations. Light cultivation. Each salt concentration combination is shown in Table 5, each growth regulator concentration combination is shown in Table 6, and each sugar concentration combination is shown in Table 7. Each treatment was inoculated with 10 stem segments, 2 stem segments / bottle, repeated 3 times, and after 60 days of cultivation, Observe the germination and growth of roots, and calculate the rooting rate, average rooting number, average root length and average plant height to determine the best rootin...

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Abstract

The invention discloses a method for tissue rapid cultivation of Acer rubrum. The method comprises the following steps: (1) conducting surface sterilization by adopting tender stem segments of Acer rubrum as explants; (2) cultivating on an initial medium for 25-30 days; (3) cultivating on a multiplying medium for 20-40 days; (4) conducting rooting cultivation on a rooting medium till generating appropriate roots. By adopting the method, excellent aseptic seedlings of Acer rubrum can be rapidly and constantly acquired, the survival rate of seedlings is high, technical guarantee is provided and solid foundation is established for industrialized seedling production of Acer rubrum, and industrialized development of Acer rubrum cultivation in China is facilitated.

Description

technical field [0001] The invention relates to a method suitable for rapid tissue propagation of American red maple. Background technique [0002] American red maple is a cultivar of Acer palmatum in the genus Aceraceae of the Aceraceae family. It is native to the east coast of the United States and has a wide range of adaptability. It can adapt to acidic soil, neutral soil and calcareous soil; Drought, humidity, and barren resistance, but likes a humid and warm climate, and grows well in a fertile soil and well-drained environment. American red maple is a large deciduous tree with 3-5 palmate leaves and 5-10cm in length. The young leaves are bright red or purple in spring, and turn slightly green in summer. The whole plant has a beautiful appearance, and the leaves often appear bright red in autumn. It is a well-known ornamental tree species for urban greening and landscape building, and is currently widely used in parks, courtyards, and community greening. The branches ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邱义兰刘如石廖旻晶
Owner HUNAN NORMAL UNIVERSITY
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