Method for producing trichosanthes tissue culture seedlings

A technique for cultivating seedlings of Trichosanthes and tissue culture, which is applied in the field of plant biotechnology tissue culture, can solve the problems of troublesome production procedures, high cost, low seedling rate and the like, so as to reduce production costs, improve seedling efficiency, and simplify production procedures. Effect

Active Publication Date: 2015-04-22
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production process is troublesome, the seedling rate is low, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Collect single-node stems with axillary buds from robust Trichosanthes plants and carry out routine disinfection and sterilization; cuttages of the above-mentioned stems were mixed with MS+LFS 0.1mg·L -1 The prepared solid medium can directly induce the normal germination and growth of 60% Trichomonas axillary bud explants after 30 days of culture without adding any other plant hormones and regulators, and then build regenerated plants with complete roots and seedlings, that is, tissue culture seedlings. The shape of stems and leaves of these regenerated plants is normal, with an average plant height of 3.8cm and an average adventitious root of 2.5 per plant. They can be used as subculture materials and continue to proliferate in the same medium without the need to prepare additional "subculture medium". It can also be transplanted out of bottles and sold, without the need to set up a "rooting program" and prepare a "rooting medium". Therefore, with MS+LFS 0.1mg·L -1 T...

Embodiment 2

[0015] Collect single-node stems with axillary buds from robust Trichosanthes plants and carry out routine disinfection and sterilization; cuttages of the above-mentioned stems were mixed with MS+LFS 0.5mg·L -1 The prepared solid medium can directly induce the normal germination and growth of 100% Trichomonas axillary bud explants after 25 days of culture without adding any other plant hormones and regulators, and then build regenerated plants with complete roots and seedlings, that is, tissue culture seedlings. The shape of stems and leaves of these regenerated plants was normal, with an average height of 5.2 cm and an average of 3.5 adventitious roots / plant. These regenerated plants can be used as subculture materials, and can be propagated in the same culture medium, without additional preparation of "subculture medium". It can also be transplanted out of bottles and sold, without the need to set up a "rooting program" and prepare a "rooting medium". Therefore, with MS+LFS...

Embodiment 3

[0017] The following is the method of the present invention, through the preferred formula MS+LFS 0.3mg L -1 The comparison of the three key stages of "initiation", "subculture" and "rooting" when the prepared single hormone solid medium and three conventional medium containing different hormones were used for the production of Trichosanthes chinensis tissue culture seedlings:

[0018] 1. "Axillary bud explant initiation" stage:

[0019] Explants: Single-node stem segments with axillary buds were collected from healthy plants and subjected to routine disinfection and sterilization. There were 30 bottles of medium in each group, and one explant was inoculated in each bottle. Eliminate polluted bottles after cultivating for 7 days, take 5 bottles at random for each group, and observe statistics: seedling rate=number of seedling explants / number of inoculated explants×100%, average plant height (from terminal bud to rooting node), average adventitious root Number (bar / plant).

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PUM

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Abstract

The invention discloses a method for producing trichosanthes tissue culture seedlings. The method comprises the following steps: (1) collecting single-node stems with axillary buds from strong trichosanthes plants, and performing conventional disinfection and sterilization; and (2) cutting the stems into an MS improved solid medium added with LFS for performing trichosanthes tissue culture seedling production, wherein the formula of the MS improved solid medium is 0.1-0.5mg.L<-1>(MS+LFS). The MS improved solid medium can induce explants to directly grow to the trichosanthes tissue culture seedlings with complete into roots and buds. The method disclosed by the invention has the advantages of simple production procedure, high tissue culture seedling quality and low cost.

Description

technical field [0001] The invention belongs to the field of plant biotechnology tissue culture, and in particular relates to a method for producing Trichosanthes tissue culture seedlings. technical background [0002] Trichosanthes kirilowii Maxim., also known as Tiangua, Diaogua, and Laoyagua, belongs to the genus Trichosanthes kirilowii Maxim. It is one of the important traditional Chinese medicinal materials in our country. It has high application value and broad development prospects in medical and health care. However, in the commercial production of its tissue culture seedlings, almost without exception, 6-BA (N 6 -BA,N 6 -benzyl adenine) and growth regulators such as IAA (indole acetic acid), NAA (naphthalene acetic acid) or IBA (indole butyric acid) in auxin (Auxin), respectively compounded into " Three different mediums are required for the three production steps of "explant start", "rootless seedling subculture propagation" and "subculture seedling rooting", an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周凤珏许鸿源龚银花梁琼月许皓翔莫淑媚
Owner GUANGXI UNIV
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