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Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture

A technology of chromosome doubling and licorice, applied in gardening methods, botanical equipment and methods, gardening, etc., can solve problems such as troublesome methods and long time-consuming, and achieve the effect of alleviating the shortage of supply

Inactive Publication Date: 2015-04-22
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are few reports on chromosome doubling of Glycyrrhiza thorns. Although Wu Yuxiang and others used the improved agar smear method and direct infiltration method to treat the terminal buds of Glycyrrhizae thorns seedlings for polyploid mutagenesis, the method was cumbersome and took a long time.

Method used

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  • Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture
  • Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture
  • Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1. Seed pre-treatment: select mature, plump, healthy and pest-free licorice seeds, soak them in 98% concentrated sulfuric acid for 40 minutes after sand milling, and disinfect them with 1% mercury liter for 10 minutes.

[0022] 2. Obtain sterile seedlings: take the mercuric-treated licorice seeds and rinse them with sterile water for 4 times, inoculate them into conical flasks with MS medium as the matrix, and culture them in dark at 25°C for 3 days, and then The light and dark were alternately treated for 4 days with 2000lux intensity of light for 10h, and then moved to light culture at a temperature of 26°C, and the plants were ready for use when they grew to 8cm.

[0023] 3. Inoculate the sterile seedlings in step 2 onto the zeatin mixed medium, place them in an artificial climate box, and culture them in the dark at 23° C. for 5 days, and then light them at 2000 lux for 16 hours every day. Its healing rate is as high as 90.45%, and its texture is loose and its growt...

Embodiment 2

[0028] 1. Seed pre-treatment: select mature, plump, healthy and pest-free licorice seeds, soak them in 98% concentrated sulfuric acid for 41 minutes after sand milling, and disinfect them with 1% mercuric chloride for 10 minutes.

[0029] 2. Obtain sterile seedlings: Take the licorice thorns seeds treated with mercuric chloride and wash them with sterile water for 3 times, inoculate them into conical flasks with MS medium as the substrate, and culture them in dark at 25°C for 3 days, and then The light and dark were alternately treated with 2000lux intensity of light for 10 hours for 4 days, and then moved to light culture at a temperature of 26° C., and the plants were used when they grew to 10 cm.

[0030] 3. Inoculate the sterile seedlings in step 2 onto the zeatin mixed medium, place them in an artificial climate box, and culture them in the dark at 23° C. for 5 days, and then light them at 2000 lux for 16 hours every day. Its healing rate is as high as 90.45%, and its tex...

Embodiment 3

[0035] 1. Seed pre-treatment: select mature, plump, healthy and pest-free licorice seeds, soak them in 98% concentrated sulfuric acid for 39 minutes after sand milling, and disinfect them with 1% mercury liter for 10 minutes.

[0036] 2. Obtain sterile seedlings: take the mercuric-treated licorice seeds and rinse them with sterile water for 4 times, inoculate them into conical flasks with MS medium as the matrix, and culture them in dark at 25°C for 3 days, and then 2000lux intensity of light for 10h was alternately treated with light and dark for 4 days, and then moved to light culture at a temperature of 26°C, and the plants were ready for use when they grew to 6cm.

[0037] 3. Inoculate the sterile seedlings in step 2 onto the zeatin mixed medium, place them in an artificial climate box, and culture them in the dark at 23° C. for 5 days, and then light them at 2000 lux for 16 hours every day. Its healing rate is as high as 90.45%, and its texture is loose and its growth con...

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Abstract

The invention discloses a method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture. Dry and mature glycyrrhiza pallidiflora maxim seeds serve as materials, and the steps of seed pretreatment, aseptic seedling obtaining, culture medium screening of best induced calluses, glycyrrhiza pallidiflora maxim callus processing through colchicines and subculture are executed. The glycyrrhiza pallidiflora maxim seeds are induced through the colchicines to double the chromosomes so as to obtain excellent seed sources of liquorice, and the situation that in the liquorice market, the demand exceeds the supply is remitted.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for doubling the chromosomes of Glycyrrhiza thorns through tissue culture. Background technique [0002] Licorice ( Glycyrrhiza pallidiflora Max.), also known as wild licorice, cocklebur, etc., is a perennial semi-shrub plant of the genus Licorice in the Fabaceae Papilionaceae. The roots and seeds of Glycyrrhiza thorns are used as medicine, which has the effects of prolactin, insecticide, antidiarrheal, and anticancer. It has long been used as one of the important plant resources to replace licorice. Over the years people have conducted extensive and in-depth research on licorice, but most of them have focused on cultivation, active ingredients and pharmacological effects. [0003] In recent years, the use of licorice in the pharmaceutical, food and cosmetic industries has increased sharply, and the medicinal materials of licorice have mainly relied on wild resources...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郭晔红杨萍
Owner GANSU AGRI UNIV
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