Method for preparing microbial food antioxidant
A food antioxidant and antioxidant technology, which is applied in the field of antioxidant preparation and microbial food antioxidant preparation, can solve the problems of improved safety, complex process and high cost, and achieve strong antioxidant capacity, promote cell metabolism, and improve safety. high sex effect
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Embodiment 1
[0016] (1) Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae, tea yeast, and Lactobacillus bulgaricus, and mix the cultured strains at a weight ratio of 25:20:15:20 to obtain a strain stock solution;
[0017] (2) Mix the stock solution of the strain and the medium at a weight ratio of 10:60, put it into a fermenter and stir evenly, and carry out airtight culture at a temperature of 25°C. After the pH value is less than 3.60, the culture time is 10 days, and then the culture After the mixture passes through the spiral sedimentation centrifuge, it enters the disc centrifuge, and the liquid obtained after centrifugation is sterilized at 100°C for 30 minutes to obtain the microbial antioxidant fermentation liquid;
[0018] (3) Mix rosemary extract, resveratrol and microbial antioxidant fermentation liquid obtained in step (2) at a weight ratio of 5:1:90, disperse at 10,000 rpm for 3 minutes using a high-speed disperser, and stand still for 1 hour to prepare The ultima...
Embodiment 2
[0020] (1) Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae, tea yeast, and Lactobacillus bulgaricus, and mix the cultured strains at a weight ratio of 30:25:20:25 to obtain a strain stock solution;
[0021] (2) Mix the stock solution of the strain and the medium at a weight ratio of 15:70, put it into a fermenter and stir evenly, and carry out airtight culture at a temperature of 30°C. After the pH value is less than 3.6, the culture time is 15 days, and then the culture After the mixture passes through the spiral sedimentation centrifuge, it enters the disc centrifuge, and the liquid obtained after centrifugation is sterilized at 110°C for 40 minutes to obtain the microbial antioxidant fermentation liquid;
[0022] (3) Mix rosemary extract, resveratrol and microbial antioxidant fermentation broth obtained in step (2) at a weight ratio of 10:2:92, disperse at 12,000 rpm for 4 minutes using a high-speed disperser, and stand still for 1 hour to prepare The ultimat...
Embodiment 3
[0024] (1) Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae, tea yeast, and Lactobacillus bulgaricus, and mix the cultured strains at a weight ratio of 35:30:25:30 to obtain a strain stock solution;
[0025] (2) Mix the stock solution of the strain and the culture medium at a weight ratio of 20:75, put it into a fermenter and stir evenly, and carry out airtight culture at a temperature of 35°C. After the pH value is less than 4.00, the culture time is 18 days, and then the culture After the mixture passes through the spiral sedimentation centrifuge, it enters the disc centrifuge, and the liquid obtained after centrifugation is sterilized at 120°C for 50 minutes to obtain the microbial antioxidant fermentation liquid;
[0026] (3) Mix rosemary extract, resveratrol and microbial antioxidant fermentation liquid obtained in step (2) at a weight ratio of 15:3:93, disperse at 13,000 rpm for 4 minutes using a high-speed disperser, and stand still for 2 hours to prepare ...
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