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Method for preparing high-solubility stevioside compound

A technology of steviol glycosides and high solubility, which is applied in the field of preparation of high-solubility steviol glycoside complexes, can solve the problems of poor water solubility and limited application, and achieves high water solubility, good biocompatibility, and improved water solubility and stability. Effect

Inactive Publication Date: 2015-04-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the structural differences of each component, their solubility properties are also quite different. For example, steviolbioside, steviol and isosteviol have poor water solubility, which limits their application in water-phase catalytic reactions and clinical applications.

Method used

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  • Method for preparing high-solubility stevioside compound
  • Method for preparing high-solubility stevioside compound
  • Method for preparing high-solubility stevioside compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] According to the phase solubility method [1] , prepare a series of HP-β-CD concentration solutions, directly add excess St; then stir at 40°C, 50°C, and 60°C respectively; take the supernatant after 24h, and measure the solubility of St by HPLC. Depend on figure 1 It can be seen that St increases linearly with the concentration of HP-β-CD. When the concentration of HP-β-CD is 40 mg / mL, the solubility of St increases by more than 2 times at the three temperatures. Judging from the phase solubility curve, the coating type belongs to A L type, indicating that St and HP-β-CD molecules form a stable complex at a molar ratio of 1:1.

Embodiment 2

[0017] Same as in Example 1, the phase solubility curve of Sbio / HP-β-CD was determined. Changes according to the retention time of the complex in HPLC (retention time advances, figure 2 ) to preliminarily judge the formation of the complex; and it can be determined that the coating type still belongs to A L Type, Sbio and HP-β-CD molecules form a stable complex with a molar ratio of 1:1. The water solubility of Sbio increased from about 0.1mg / mL to 9mg / mL.

Embodiment 3

[0019] Hydroxypropyl-β-cyclodextrin was prepared into a 100mg / mL aqueous solution, stevioside was dissolved in ethanol, the molar ratio of stevioside to HP-β-CD was 1:1, and then slowly added dropwise to HP-β- In CD aqueous solution; continuously stir at 45°C for 24h, then continue to stir at room temperature for 6h; filter, and vacuum dry at 60°C until constant weight to obtain the stevioside complex. The solubility of the obtained St / HP-β-CD complex was increased by 10 times. The 10mg / mL St complex was placed at 25°C for 30 days without obvious precipitation. The uncoated St had precipitation after 1 hour under the same conditions. .

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Abstract

The invention discloses a method for preparing a high-solubility stevioside compound and belongs to the field of natural compound modification. According to the method, a corresponding high-solubility stevioside compound can be prepared by taking hydroxypropyl-beta-cyclodextrin (HP-beta-CD) as a matrix and stevioside molecules as an object through simple dissolution, coating, filtration and drying. The method has the technical effects that the solubility and the stability of stevioside in water are greatly improved, so that orally taken preparations and preparations for injection can be easily prepared.

Description

technical field [0001] The invention belongs to the technical field of natural compound modification, and in particular relates to a preparation method of a high-solubility steviol glycoside complex. Background technique [0002] Stevia is a perennial herbaceous plant with perennial roots in the Asteraceae family. The indigenous people of South America have begun to use the leaves of this plant as a sweetener hundreds of years ago. Steviol glycosides are a class of natural sweeteners with high sweetness and low calorie value, known as the "third sugar source" after sugarcane and sugar beet. From 2008 when the FDA approved rebaudioside A (Reb A) in stevioside as a GRAS substance, to 2011 when the European Commission finally approved that stevioside can be used as a food additive, stevioside has been widely recognized and used , and my country has become the world's largest producer of stevia, accounting for about 80% of the world's share. Toxicology experiments show that ste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/00C08L5/16C08K5/1545A23L1/09A23L29/30
Inventor 万会达夏咏梅方云李丹覃晓雨
Owner JIANGNAN UNIV