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Tree peony bark medicinal material based on activity screening and preparation detecting method

A detection method and technology of Cortex Moutan, applied in the field of Chinese medicine research methodology, can solve problems such as inability to objectively and accurately reflect the quality of Cortex Moutan medicinal materials.

Inactive Publication Date: 2015-04-22
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the quality control of Cortex Moutan and its products, the 2010 "Chinese Pharmacopoeia" quality control standard stipulates that the medicinal materials contain paeonol not less than 1.2%. This quality control standard cannot objectively and accurately reflect the quality of Cortex Moutan.

Method used

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  • Tree peony bark medicinal material based on activity screening and preparation detecting method
  • Tree peony bark medicinal material based on activity screening and preparation detecting method
  • Tree peony bark medicinal material based on activity screening and preparation detecting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This technology establishes a solid-phase screening system for mesangial cell membranes, and uses LC / ESI / MS / MS technology to find, identify and assign effector marker peaks, and finally finds that 8 substances in paeonol are binding components to mesangial cells , the specific test methods and results are as follows:

[0035] 1. Cell membrane solid-phase paeonol product detection

[0036] (1) Instruments and reagents: Agilent 1200 high-performance liquid chromatography (DAD detector); XW-80A micro-vortex mixer (Shanghai Huxi Analytical Instrument Factory Co., Ltd.); nitrogen blowing instrument (Organomation, USA). Mass spectrometer (USA). Methanol, acetonitrile, formic acid (chromatographically pure, USA, TEDIA), double distilled water, and other reagents were all of domestic analytical grade.

[0037] (2) Effect components in Cortex Moutan of mesangial cell membrane immobilization: take human mesangial HBZY-1 cells, make it adhere to the wall and grow normally, remov...

Embodiment 2

[0045] This technology establishes a non-enzymatic glycosylation system for the generation of advanced glycation end products (AGEs) in vitro, and uses LC / ESI / MS / MS technology to find, identify and assign effector marker peaks. It was found that after the paeonol extract was reacted with methylglyoxal, some chromatographic peaks disappeared or decreased, and some chromatographic peaks also increased. Paeoniflorin. The specific test methods and results are as follows:

[0046] Catching carbonyl compound active species identification and detection

[0047] (1) Instruments and reagents: Agilent 1200 high-performance liquid chromatography (DAD detector); XW-80A micro-vortex mixer (Shanghai Huxi Analytical Instrument Factory Co., Ltd.); nitrogen blowing instrument (Organomation, USA). Methanol, acetonitrile, formic acid (chromatographically pure, USA, TEDIA), double distilled water, and other reagents were all of domestic analytical grade.

[0048] (2) Cortex Moutan extract and ...

Embodiment 3

[0056] This technology establishes a platelet solid-phase chromatography system, and uses LC / ESI / MS / MS technology to find, identify and assign effect substance marker peaks. It was found that after the paeonol extract reacted with platelets, 4 components existed in the dissociated solution and exerted their effects. The screened active substances are oxidized paeoniflorin, tetragalloyl glucose, pentagalloyl glucose and benzoyl paeoniflorin. The specific test methods and results are as follows:

[0057] Detection of active substances that inhibit platelet aggregation

[0058] (1) Instruments and reagents: Agilent 1200 high-performance liquid chromatography (DAD detector); XW-80A micro-vortex mixer (Shanghai Huxi Analytical Instrument Factory Co., Ltd.); nitrogen blowing instrument (Organomation, USA).

[0059] Methanol, acetonitrile, formic acid (chromatographically pure, USA, TEDIA), double distilled water, and other reagents were all of domestic analytical grade.

[0060] ...

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Abstract

The invention provides a method for controlling quality of multiple components of a tree peony bark medicinal material, an intermediate and a preparation product. The method comprises the following steps: screening out components and grouping the components through a cell membrane solid-phase chromatography technology and a glycosylation incubation system; on the basis of determining the six main components which are oxypaeoniflorin, paeoniflorin, trigalloyl glucose, pentagalloyl glucose, benzoyl-paeoniflorin and paeonol by combining cluster analysis with main component analysis, establishing a content synchronous measurement method for oxypaeoniflorin, paeoniflorin, trigalloyl glucose, pentagalloyl glucose, benzoyl-paeoniflorin and paeonol in the tree peony bark medicinal material, a tree peony bark extract intermediate and the tree peony bark preparation product by adopting the same liquid-phase chromatographic condition, and determining the component composition structure ratio range as follows: (1.3-1.5):(10.4-13.9):0.5:(2.7-3.5):(1.0-1.2):(14.9-16.1).

Description

technical field [0001] The invention relates to a method for detecting cortex moutan medicinal materials and preparations based on activity screening, and belongs to the field of research methodology of traditional Chinese medicine. Background technique [0002] At present, the quality control method of Chinese medicinal materials, intermediates and preparations at home and abroad is to establish a qualitative and quantitative analysis method for one or two active ingredients (main components) in Chinese medicine on the basis of chemical composition research, and then control the quality of Chinese medicine. . However, this quality control method fails to reflect the overall characteristics of traditional Chinese medicine, and fails to provide evidence related to these quality control indicators and drug efficacy. What is more worthy of attention is that the quality control of these ingredients is not as high as possible, but different ingredients. It should be controlled w...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 封亮贾晓斌张明华顾俊菲汪春飞
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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