Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular identification method for zero-type fruit branch genes of cotton

A zero-type fruit branch and molecular identification technology, applied in the biological field, can solve the problems of speeding up the breeding and improvement of zero-type fruit branch products, poor accuracy, and high consumption, so as to speed up the breeding and improvement process, with high accuracy and speed fast effect

Active Publication Date: 2015-04-29
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
View PDF1 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems in the prior art that the field identification and detection method of cotton strains containing the zero-type fruiting branch gene takes a long time, consumes a lot, and has poor accuracy, and provides a molecular identification method for the zero-type fruiting branch gene. Thereby, the breeding and improvement process of zero-type fruiting branch strains can be accelerated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular identification method for zero-type fruit branch genes of cotton
  • Molecular identification method for zero-type fruit branch genes of cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with the examples.

[0037] Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased.

[0038] Tris-Cl: Tris(hydroxymethyl)aminomethane-HCl]; EDTA: Ethylenediaminetetraacetic acid; SDS: Sodium dodecyl sulfate; PVP: Polymer Polyvinylpyrrolidone

[0039] All reagents were purchased from: Sangon Bioengineering (Shanghai) Co., Ltd.; purity: analytically pure.

[0040] 1 DNA extraction

[0041] A total of 32 mat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biotechnology, relates to a molecular identification method for zero-type fruit branches of cotton, and particularly provides a gene specific primer marker used for identifying a cotton strain with zero-type fruit branch genes. Nucleotide sequences are as shown in SEQ ID No.1 and SEQ ID No.2. An individual strain carrying the zero-type fruit branch genes in a segregation population is identified by the gen specific marker. The method is high in identification speed, high in accuracy, simple to carry out and very suitable for indoor identification and molecular marker-assisted selection of the zero-type branches of the cotton.

Description

Technical field [0001] The present invention is a biotechnology field, which involves a molecular identification method for a zero -type fruit branches gene appraisal and molecular marking auxiliary breeding. Background technique [0002] Zero fruit branches refer to a variant of cotton fruit branches as limited fruit branches.It shows that cotton peaches are born directly in the armpits of the main stems and leaves, or only one fruit.Due to the growth characteristics of zero -type fruit branches, after cotton, nutrition growth is basically stopped, which can reduce the management of cotton pruning management.At the same time, due to the growth and development of most of the cotton bells, cotton bells are developing rapidly, mature early, and cotton peaches in the upper and lower parts of a single plant have better maturity and consistency, which is conducive to the harvest of cotton.The cotton bell of the zero -type fruit branches has a reasonable spatial distribution. The cotto...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈伟张永山姚金波李燕褚丽刘海菊
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com