Porcine UNC13B gene SNP (single nucleotide polymorphism) marker related to porcine eye muscle area character

A marker, pig eye muscle technology, applied in the field of genetic marker detection, can solve the problem of no correlation report between single nucleotide polymorphism and eye muscle area

Active Publication Date: 2015-04-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The UNC13B gene is expressed in renal cortical epithelial cells and upregulated in hyperglycemic cells. GO annotations on this gene include diacylglycerol binding activity and signal transduction activity, but there is no single nucleotide polymorphism on this gene so far A report on the correlation between sex and eye muscle area

Method used

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  • Porcine UNC13B gene SNP (single nucleotide polymorphism) marker related to porcine eye muscle area character
  • Porcine UNC13B gene SNP (single nucleotide polymorphism) marker related to porcine eye muscle area character
  • Porcine UNC13B gene SNP (single nucleotide polymorphism) marker related to porcine eye muscle area character

Examples

Experimental program
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Effect test

Embodiment 1

[0029] This example is the use of the Fludigm genotyping method to detect different individual genotypes of Duroc and the correlation analysis with eye muscle area traits.

[0030] 1. Test material

[0031] 200 Duroc F2 resource populations in Dalang original seed farm.

[0032] 2. Experimental reagents

[0033] The genome extraction kit was purchased from Promega, the PCR preamplification enzyme kit was purchased from Qiagen, the genotyping chip and genotyping kit were purchased from Fludigm, the genotyping PCR enzyme kit was purchased from Biotium, and the dye ROX was purchased from Invitrogen.

[0034] 3. Genomic DNA extraction

[0035] Duroc pig ear tissue was digested with tissue extract and proteinase K overnight, extracted with Promega’s DNA Purification Kit, dissolved in ddH2O, adjusted to a final concentration of 80-120 ng / μl, and stored at -20°C.

[0036] 4. PCR pre-amplification

[0037] The pre-amplification reaction system is:

[0038]

[0039] The pre-amp...

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Abstract

The invention relates to a porcine UNC13B gene SNP (single nucleotide polymorphism) marker related to the porcine eye muscle area character. The marker is mutation of a UNC13B (Sscrofa 10.2) gene chr1:263861722 locus T-C. The invention also provides specific primers for detecting the SNP marker. By utilizing a method provided by the invention, the porcine eye muscle area character can be obtained by judging porcine UNC13B gene SNP so as to provide guidance for the breeding work of pigs and select TT-type pigs with the maximum porcine eye muscle area to carry out breeding and culturing.

Description

technical field [0001] The invention relates to the field of genetic marker detection in molecular genetics, in particular to a pig UNC13B gene SNP marker related to pig eye muscle area traits. Background technique [0002] my country is a large pig-raising country, and the market demand for pork production and quality is increasing day by day. Increasing pork production and improving pork carcass quality have become the work that breeding scientists have been exploring for a long time. Early breeding efforts were mainly focused on phenotypic selection of pigs. With the continuous advancement of genomic work and the extensive development of genetic markers, molecular selection is gradually becoming a reliable and effective selection method, combining phenotypes with population-wide genome-wide association of genetic markers Analysis is widely used. [0003] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) refers to the existence of two or more different ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 胡晓湘方素云王润铭杨瑞飞张苏红李宁
Owner CHINA AGRI UNIV
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