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Protein and nucleic acid separation device and method

A nucleic acid separation and protein technology, applied in biochemical equipment and methods, microbial determination/inspection, preparation of samples for testing, etc. question

Active Publication Date: 2015-04-29
BEIJING INSTITUTE OF TECHNOLOGYGY
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AI Technical Summary

Problems solved by technology

However, these separation operations often affect or even destroy the activity of another biomacromolecule when separating one biomacromolecule.
Moreover, these methods usually require relatively complicated manual operations or the use of multiple instruments and equipment, and these operations also have many shortcomings in terms of time and separation efficiency.
[0003] Therefore, in the field of separation and purification of biomacromolecules, there is still a lack of a miniaturized and automated separation device for the whole protein and nucleic acid of cells.

Method used

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  • Protein and nucleic acid separation device and method
  • Protein and nucleic acid separation device and method

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Embodiment Construction

[0027] Embodiments of the present invention are described below with reference to the drawings. Elements and features described in one drawing or one embodiment of the present invention may be combined with elements and features shown in one or more other drawings or embodiments. It should be noted that representation and description of components or processes that are not relevant to the present invention and known to those of ordinary skill in the art are omitted from the drawings and descriptions for the purpose of clarity.

[0028] An embodiment of the present invention provides a protein-nucleic acid separation device, which includes: a macromolecule sample storage area, a buffer storage area, a free-flow electrophoresis chip, a protein collection area, a nucleic acid collection area, and a power supply.

[0029] Wherein, the free-flow electrophoresis chip is provided with an electrophoresis channel inside, and electrodes are arranged on both sides of the electrophoresis ...

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Abstract

The invention relates to the field of biological equipment manufacturing engineering. A protein and nucleic acid separation device comprises a macromolecular sample storage area, a buffer solution storage area, a free flow electrophoresis chip, a protein collection area, a nucleic acid collecting area and a power supply, wherein an electrophoresis channel is formed inside the free flow electrophoresis chip; electrodes are arranged on the two sides of the electrophoresis channel; positive and negative electrodes of the power supply are respectively connected to the electrodes on the two sides; a sample inlet and a buffer solution inlet are formed in the inflow end of the electrophoresis channel; a protein outlet and a nucleic acid outlet are respectively formed in positions, close to the electrodes on the two sides, at the outflow end of the electrophoresis channel; the sample inlet is connected with the macromolecular sample storage area; the buffer solution inlet is connected with the buffer solution storage area; the protein outlet is connected with the protein collecting area; the nucleic acid outlet is connected with the nucleic acid collecting area. The protein and nucleic acid separation device provided by the invention is compact and simple in structure, and easy to operate, and can realize efficient and fast separation of protein from nucleic acid.

Description

technical field [0001] The invention relates to the field of biological equipment manufacturing engineering, in particular to a protein and nucleic acid separation device and method. Background technique [0002] In the current field of life science research, two kinds of biomacromolecules, protein and nucleic acid, are important research objects. Protein is responsible for various physiological functions, maintains the metabolic activities of organisms as a whole, and is the direct expresser of biological traits, while nucleic acid is the main substance that reveals the inheritance and variation of life. In the analysis and research of biological samples, the samples often contain a large amount of protein and nucleic acid, which requires separation and purification of the protein or nucleic acid. There are many separation and purification methods for biological macromolecules, such as traditional dialysis, ultrafiltration, coagulation, etc. Gel filtration, organic precipi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34C12Q1/68
Inventor 吕芳石宇代胜平戴荣继邓玉林
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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