A protein nucleic acid separation device and method

Abstract

The invention relates to the field of biological equipment manufacturing engineering. A protein and nucleic acid separation device includes: a macromolecule sample storage area, a buffer storage area, a free-flow electrophoresis chip, a protein collection area, a nucleic acid collection area, and a power supply; the free-flow electrophoresis chip An electrophoresis channel is arranged inside, and electrodes are arranged on both sides of the electrophoresis channel, and the positive and negative poles of the power supply are respectively connected to the electrodes on both sides; the inflow end of the electrophoresis channel is provided with a sample inlet and a buffer inlet, and the electrophoresis channel A protein outlet and a nucleic acid outlet are respectively set at the outflow end of the outlet near the electrodes on both sides; the sample inlet is connected to the macromolecule sample storage area, and the buffer inlet is connected to the buffer storage area; the protein outlet is connected to the A protein collection area, the nucleic acid outlet is connected to the nucleic acid collection area. The protein-nucleic acid separation device of the present invention has a compact and simple structure, is easy to operate, and can realize efficient and rapid separation of protein and nucleic acid.

Description

technical field [0001] The invention relates to the field of biological equipment manufacturing engineering, in particular to a protein and nucleic acid separation device and method. Background technique [0002] In the current field of life science research, two kinds of biomacromolecules, protein and nucleic acid, are important research objects. Protein is responsible for various physiological functions, maintains the metabolic activities of organisms as a whole, and is the direct expresser of biological traits, while nucleic acid is the main substance that reveals the inheritance and variation of life. In the analysis and research of biological samples, the samples often contain a large amount of protein and nucleic acid, which requires separation and purification of the protein or nucleic acid. There are many separation and purification methods for biological macromolecules, such as traditional dialysis, ultrafiltration, coagulation, etc. Gel filtration, organic precipi...

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Problems solved by technology

However, these separation operations often affect or even destroy the activity of another biomacromolecule when separating one biomacromolecule.

Moreover, these methods usually require relatively complicated manual operations or the use of multiple instruments and equipment, and these operations also have many shortcomings in terms of time and separation efficiency.

[0003] Therefore, in the field of separation and purification of biomacromolecules, there is still a lack of a miniaturized and automated separation device for the whole protein and nucleic acid of cells.

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