A kind of refining method of organic acid
A refining method and organic acid technology, which is applied in the field of obtaining high-purity organic acids, can solve the problems of complex operation process, large solvent consumption, and inability to obtain it, and achieve the effects of simple process, low material consumption, and low impurity content
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Embodiment 1
[0033] Take 200ml and get C by fermenting Candida tropicalis 12 h 22 o 4 Organic acid fermentation broth, the concentration is 160g / l. heated to 80°C with H 2 SO 4 Adjust the pH value to 3.0, let it stand for 2 hours, and remove the liquid wax remaining in the solution to obtain the C containing bacteria.12 h 22 o 4 Organic acid crystallization solution.
[0034] C 12 h 22 o 4 Organic acid crystallization solution and 4-heptanone (4-heptanone and C 12 h 22 o 4 The weight ratio is 6:1) and mixed, heated to 115°C. After the organic acid is dissolved, the mixed system is sent to the supergravity equipment heated to 115°C for supergravity treatment. The supergravity level is 50g, and the supergravity treatment The average residence time is 0.2s, and the mixed system treated by supergravity is stilled for 60min, and the lower aqueous phase is separated; then 30ml of deionized water is added to the solvent phase to wash the organic phase, and the aqueous phase is separat...
Embodiment 2
[0036] Take 200ml and get C by fermenting Candida tropicalis 12 h 22 o 4 Organic acid fermentation broth with a concentration of 162g / l, adjust the pH to 10.0, heat to 80°C, let it stand for 2 hours, remove the liquid wax remaining in the solution, use microfiltration equipment to filter the lower layer solution to remove impurities such as bacteria to obtain a clear liquid, Add acid to the obtained clear liquid to adjust the pH value to 3.5, and heat to 80°C to obtain organic acid crystal liquid.
[0037] Put the obtained C 12 h 22 o 4 Organic acid crystallization solution and isobutyl acetate (isobutyl acetate and C 12 h 22 o 4 The weight ratio is 4:1) and mixed, heated to 95°C. After the organic acid is dissolved, the mixed system is sent to the supergravity equipment heated to 95°C for supergravity treatment. The supergravity level is 150g. The average residence time is 0.8s, and the mixed system treated by supergravity is static for 30min, and the lower aqueous ph...
Embodiment 3
[0039] Take 200ml and get C by fermenting Candida tropicalis 13 h 24 o 4 Organic acid fermentation broth with a concentration of 154g / l. Heat to 70°C, filter through a ceramic microfiltration membrane, remove bacteria and residual liquid wax to obtain a filtrate. Add H to the filtrate 2 SO 4 Adjust the pH to 2.4 and heat to 95 °C to give C 13 h 24 o 4 The organic acid crystallization solution was cooled to room temperature and filtered to obtain an organic acid filter cake.
[0040] Put the obtained C 13 h 24 o 4 Organic acid filter cake with dodecane (dodecane with C 13 h 24 o 4 The weight ratio is 8:1) and mix, heat the system to 98°C, after the organic acid is dissolved, send the mixed system into the supergravity equipment that has been heated to 98°C for supergravity treatment, the supergravity level is 300g, the supergravity The average residence time of the treatment is 5s, and the mixed system treated by supergravity is static for 40min, and the lower aqu...
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