A method for rapid separation and screening of cellulolytic fungi

Abstract

The invention discloses a method for quickly separating and screening cellulose decomposing fungi. The method comprises the following steps: A, performing selective enrichment culture and primary screening; B, performing secondary screening by a porous cell culture plate; C, performing secondary screening by plate culture; D, performing strain preservation. A cellazyme is adopted as a unique carbon source of a screening indicator and a screening culture medium, and selective pressure (including low nutrition, low pH (potential of hydrogen), a fungus inhibitor, a microelement and the like) of the culture medium is increased to quickly and accurately separate and screen out high-producing strains producing cellulase from various complicated samples; the screening indicator with sensitive response and the screening culture medium with high selectivity are adopted and the porous culture plate is applied, so that screening work is quick and accurate, and a large amount of manpower and material resources are saved.

Description

technical field [0001] The invention belongs to the fields of bioengineering and transformation and utilization of biological resources, and relates to a screening method for cellulolytic fungi. Specifically, the present invention relates to a method for rapidly separating and screening cellulolytic fungi. Background technique [0002] Cellulose is the most abundant renewable resource in nature. It is estimated that 10% of cellulose can be synthesized every year through photosynthesis. 11 ~10 12 Ton. Degrading straw cellulose into fermentable sugars, and further converting them into ethanol, single-cell protein, and gas fuels, is of great significance to solving the problems of energy crisis, food shortage, and environmental pollution faced by the world, and has become a hot spot of concern in the world. [0003] Cellulose is a high polymer composed of D-glucose molecules with β-1,4-glycosidic bonds. Its structure is stable and not easy to degrade. At present, the difficu...

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Problems solved by technology

[0007] The defective that existing technology exists is: on Congo red preliminary screening plate culture medium, the bacterial colony of fungus easily overgrows plate and influences the observation of hydrolysis zone; Bacteria rapid growth influences the separation of target bacterium on plate culture medium; The cellulose (polysaccharide substance) in the mixture forms a red complex, and can also form a red complex with starch, so the amylase-producing strains can also interfere with the screening results; the strains obtained from the primary screening of Congo Red need to be tested for the disintegration of filter paper. This experiment is to visually observe the disintegration effect of cellulose filter paper, because the cellulose filter paper will soften and swell in the liquid medium, and it will be easily broken after shaking, thus affecting the judgment of the decomposition effect of cellulase

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