Fluorescence imaging device and method

Abstract

The invention relates to a fluorescence imaging device and method. The device comprises at least one group of laser sources, a to-be-tested sample, an optical color filter, an imaging lens, an image sensor and an intelligent terminal. An optical filter is positioned above the to-be-tested sample and fixedly arranged on the lower end of the imaging lens, the image sensor is fixedly arranged on the upper end of the imaging lens and connected with the intelligent terminal; the laser sources emit laser to be irradiated onto the to-be-tested sample and generate fluorescence which is irradiated onto the optical color filter, only fluorescence with specific wavelength in the fluorescence can penetrate through the optical color filter and irradiate into the imaging lens, and is focused on the image sensor by the imaging lens. According to the fluorescence imaging device, the image acquiring speed and the irradiation uniformity of the laser excited light can be improved, the stability and reliability of equipment can be improved, the cost can be effectively lowered, the utilization rate of the light source can be increased, and the heat radiation characteristic can be greatly improved.

Description

technical field [0001] The invention relates to the field of optical imaging, in particular to a fluorescence imaging device and method. Background technique [0002] Due to the single-spectrum characteristics of the laser, the light of the appropriate wavelength emitted by the laser light source is the most ideal excitation light source for fluorescence detection. However, due to the high orientation of the laser light source itself and the inability to uniformly illuminate large-area samples, the current application of laser light sources in the field of fluorescence detection is limited to single-point detection or linear detection. Since single-point detection or linear detection can only detect one point or one line at a time, current detection systems of this type must use highly complex motion mechanisms to obtain two-dimensional fluorescence images of the sample to be tested. Therefore, due to the existence of the moving structure, the overall structure of this type...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a fluorescent imaging device and method, which solves the problem of complex structure, difficult installation and debugging, slow image acquisition speed, high cost, low light source utilization rate and heat dissipation of the traditional large-area non-scanning fluorescent imaging system. Defects such as poor characteristics

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