Intra-embryonic provision tonic capable of promoting growth of broiler chicken and application of intra-embryonic provision tonic
An intra-embryonic feeding and broiler technology, which can be used in medical preparations containing active ingredients, drug delivery, organic active ingredients, etc., and can solve the problem that the synthetic amount cannot meet the needs of the body.
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Embodiment 1
[0034] ——Preparation of Intraembryo Supplies of the Present Invention
[0035] Pyrroloquinoline quinone disodium (gifted by Shanghai Medical Life Science Research Center, produced by chemical synthesis) was heated and dissolved in sterile normal saline (0.85% NaCl solution) at 65°C at a concentration of 2mM in a light-proof environment, and then micro- Store in the dark after sterilizing the pore filter.
Embodiment 2
[0037] ——Injection method of pyrroloquinoline quinone for intra-embryo feeding (intragas injection)
[0038] The operation steps of the injection method of feeding pyrroloquinoline quinone are as follows: when broiler embryos are hatched on the 7th day of embryo incubation, move the hatched embryos to the operating table, use a pencil to quickly trace the outline of the air cells of the embryo eggs, and use 75% alcohol to infiltrate the egg. Wipe and disinfect the absorbent cotton ball near the injection point, drill a hole with a micro-drill at the top of the air chamber, inject 1ml with a needle length of 8mm continuous syringe and immediately seal it with paraffin. The whole process is completed within 15 minutes and the eggs are returned to the incubator to continue incubation.
Embodiment 3
[0040] ——Injection method of pyrroloquinoline quinone for intra-embryo feeding (amniotic injection)
[0041] On the 17.5th day of broiler embryo hatching, move the hatched embryo to the operating table, use an egg canister to illuminate the position of the embryo, determine the position of the amniotic cavity and use a pencil to draw a small circle at the position of the needle, and use a cotton ball soaked with 75% alcohol on the Wipe and disinfect near the injection point, drill a hole with a micro-driller at the needle insertion position, inject 1mL with a disposable syringe with a needle length of 15mm, and seal it with paraffin immediately. The whole process is completed within 15 minutes and the eggs are returned to the incubator Continue to hatch.
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