Method for manufacturing schizophyllum commune mycelium pellets and method for producing beta-glucan by using same
A manufacturing method, the technology of schizophyllum, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to form compactness, produce β-glucan, excellent particles, etc., and achieve tightness The effect of solidity increase
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Embodiment 1
[0026] Embodiment 1: the manufacture of Schizophyllum mycelium ball
[0027] For general YMG liquid medium (0.4% (w / v) yeast extract, 1% (w / v) malt extract, 0.4% (w / v) glucose) and 0.5% added to the above general YMG liquid medium (w / v) media made of vermic ulite or illite were each inoculated with Schizophyllum inoculum and mycelium liquid culture was performed.
[0028] For liquid culture, 100 ml of the liquid culture medium produced above was poured into 250 ml Erlenmeyer flasks, sterilized for use, and shaken at 150 rpm at 24° C. for five days. Thereafter, the generated particles were observed with the naked eye, and the cross-section was observed with a dissecting microscope.
[0029] As a result of the experiment, it was confirmed that the granule mycelium of Schizophyllum cultured in a medium to which clay minerals (vermiculite or illite) was added compared to the granule mycelium of Schizophyllum cultured in a general medium. Better firmness ( figure 1 , figure 2 ...
experiment example 1
[0030] Experimental Example 1: Filtration Efficiency Measurement of Schizophyllum Mycelium Balls
[0031] In this experimental example, it is intended to confirm the filtration efficiency of Schizophyllum mycelium balls produced by culturing in a medium added with the clay mineral (vermiculite or illite) of the present invention.
[0032] The mycelium culture was carried out according to the method described in the above-mentioned Example 1, and the filtration efficiency was measured based on 100 L of the culture solution, and the filtration rate and the number of filter replacements were measured. The filtration rate is converted into a percentage based on the time to measure the amount obtained per liter based on the common mycelium culture solution of Schizophyllum. Regarding the number of times of filter replacement, the number of times of replacement of a 0.45 μm filter (filter) when filtering 100 L was measured.
[0033] As a result of the experiment, it can be confirme...
Embodiment 2
[0035] Example 2: Recovery of β-glucan from Schizophyllum mycelium
[0036] In this example, the amount of β-glucan (β-glucan) produced from the Schizophyllum mycelia cultured in a medium added with clay minerals (vermiculite or illite) was intended to be confirmed.
[0037] Mycelium culture was carried out according to the method described in the above-mentioned Example 1, and regarding β-glucan, after killing microorganisms by sterilization, the mycelium was separated with a 0.45 μm filter. The culture medium was separated. Thereafter, the same amount of 95% ethanol as that of the filtered culture solution was added, and the β-glucan was precipitated while stirring at about 400 rpm, and the obtained β-glucan was dried at 65°C for 48 hours and then measured up the weight.
[0038] Experimental results, such as Figure 4As shown, it was confirmed that illite (illite) increased by about 18% and vermiculite (vermiculite) increased by about 30% compared with the culture soluti...
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