A method for preserving desoxyribonucleic acid (DNA)
A technology of deoxyribonucleic acid and collection department, which is applied in the field of prolonging the storage time of deoxyribonucleic acid, can solve the problems of sample remaining, affecting the concentration of collection, affecting the content of deoxyribonucleic acid, etc., to reduce discomfort, increase surface area, and avoid degradation Effect
Inactive Publication Date: 2015-06-03
TCI GENE
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Problems solved by technology
[0003] During the transportation process, the collection rod stained with the subject's tissue will collide with the bottle body and affect the collection concentration, and storing the DNA in a dry manner will cause degradation of the DNA and affect the accuracy of subsequent detection
[0004] In addition, the affinity between the collection stick and the collected sample will affect the content of DNA dissolved in the subsequent processing solution
For example, when the adhesion of the sample to the collection rod is greater than the force between the sample and the subsequent processing solution, a large amount of sample will remain on the collection rod; It may even affect the degree of degradation of the sample, thereby affecting the quality of DNA in the sample
Method used
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Effect test
Embodiment 1
[0024] The impact of the collection part type, temperature and time of embodiment 1 collection rod on deoxyribonucleic acid concentration
[0025] After gently scraping the oral mucosa of the subject with the collection part of the collection stick several times (preferably 6 to 7 times), the collection part containing the subject's DNA was placed in a desiccant (desiccant, purchased at isohelix company), storage bottles containing buffer or no treatment at all (as a control group), and the above containers, storage bottles or control groups were placed at low temperature (4°C), room temperature (25°C) and different storage days. (2 days, 4 days, 8 days), and then extract deoxyribonucleic acid with the following method.
[0026] Put the collection part containing the subject's deoxyribonucleic acid into a microcentrifuge tube (microcentrifuge tube) containing 500 microliters (μL) of GST buffer and 20 μL of proteinase k (proteinase k), and after 10 minutes at 60 ° C, the Take ...
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The invention provides a method for preserving desoxyribonucleic acid (DNA). The method comprises the steps of preparing a collecting stick with a collecting part with a smooth surface, the collecting part stained with DNA, and putting the collecting stick into a storage bottle filled with a buffer solution to prolong the preservation time of the DNA. The buffer solution contains ethylenediamine tetraacetic acid (EDTA) with the concentration between 20mmol (mM) and 60mM. The method for preserving desoxyribonucleic acid (DNA) is characterized in that the EDTA having a concentration within a certain range is used for preventing the degradation of DNA and the preservation time of DNA is prolonged through the selective application of the collecting stick and the buffer solution, so that the test accuracy is improved.
Description
technical field [0001] The invention relates to a method for preserving deoxyribonucleic acid, especially a method for placing a gene collection stick containing deoxyribonucleic acid in a preservation bottle to prolong the preservation time of deoxyribonucleic acid. Background technique [0002] The common method of gene collection is to collect the subject's deoxyribonucleic acid (DNA) with a collection stick, and then put the collection stick stained with the subject's DNA into an empty storage bottle or a storage bottle with a desiccant. [0003] During the transportation process, the collection rod stained with the subject's tissue will collide with the bottle and affect the collection concentration, and storing the DNA in a dry manner will cause degradation of the DNA and affect the accuracy of subsequent detection. [0004] In addition, the affinity between the collection stick and the collected sample will affect the content of deoxyribonucleic acid dissolved in the ...
Claims
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Inventor 施惠馨林咏翔关淑君黄雪茵蔡岳颖
Owner TCI GENE