Method for detecting 17bata-estradiol by using resonance scattering spectrometry based on functional nucleic acids
A resonance scattering, functional nucleic acid technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of prone to false positives, long detection time, high cost, and achieve the effect of significant technological progress, low cost, and good stability.
Inactive Publication Date: 2015-06-03
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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The invention discloses a method for detecting 17bata-estradiol by using resonance scattering spectrometry based on functional nucleic acids. The method comprises the following steps: preparing an aptamer-nanogold probe sequence, wherein the aptamer is a section of functional nucleotide sequence, and the nucleotide sequence is 5'-GCT-TCC-AGC-TTA-TTG-AAT-TAC-ACG-CAG-AGG-GTA-GCG-GCT-CTG-CGC-ATT-CAA-TTG-CTG-CGC-GCT-GAA-GCG-CGG-AAG-C-3'; combining nanogold with the aptamer to form a stable aptamer-nanogold probe; and adding a to-be-detected aqueous solution which is suspected to contain 17bata-estradiol to a probe solution, performing incubation, adding a sodium chloride solution, and judging the content of 17bata-estradiol in the to-be-detected solution by measuring the strength of a resonance scattering signal. According to the method disclosed by the invention, the detection of 17bata-estradiol can be achieved by comparing the changes of resonance scattering values.
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Estradiols17 beta estradiol +10
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Property | Measurement | Unit |
Concentration | 10.0 | mmol/l |
tensile | MPa | |
Particle size | Pa | |
strength | 10 |
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