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Multi-photon excited multidirectional lighting microscopy imaging system

A technology of microscopic imaging and imaging system, applied in the optical field, can solve the problems of slow imaging speed and achieve the effect of improving the imaging speed

Inactive Publication Date: 2015-06-03
INST OF AUTOMATION CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The invention provides a multi-photon excitation multi-directional illumination microscopic imaging system with fast imaging speed, which is used to solve the technical defect of slow imaging speed in the existing microscopic imaging technology

Method used

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  • Multi-photon excited multidirectional lighting microscopy imaging system

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Embodiment Construction

[0021] figure 1 It is a structural block diagram of Embodiment 1 of the real multi-photon excitation multi-directional illumination micro-imaging system of the present invention, figure 2 It is a structural diagram of a multi-photon excitation multi-directional illumination micro-imaging system according to an embodiment of the present invention, such as figure 1 and figure 2 As shown, the multi-photon excitation multi-directional illumination microscopic imaging system of the present invention includes: a multi-photon laser unit 1, an illumination unit 2, and an imaging detection unit 3 located on the fluorescence optical path of the imaging system from front to back;

[0022] The multiphoton laser unit 1 is used to generate and modulate laser light and send the modulated laser light into the illumination unit; the illumination unit 2 is arranged on the side of the sample to be detected, and is used to receive the modulated laser light and then Generate multidirectional l...

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Abstract

The invention discloses a multi-photon excited multidirectional lighting microscopy imaging system. From front to back, the microscopy imaging system comprises a multi-photon laser unit, a lighting unit and an imaging detection unit which are sequentially arranged on a fluorescent light path of the imaging system, wherein the multi-photon laser unit is used for generating and modulating laser and for transmitting the modulated laser to the lighting unit; the lighting unit is used for receiving the modulated laser, generating multidirectional light sheet illumination and exciting a to-be-detected sample to generate fluorescent light; and the imaging detection unit is used for detecting the fluorescent light generated from the to-be-detected sample and converting the fluorescent light into a digital image. The multi-photon excited multidirectional lighting microscopy imaging system disclosed by the invention can image rapidly, so as to image for the dynamic development of living organisms or record neuronal activities.

Description

technical field [0001] The invention belongs to the field of optics, in particular to fluorescence microscopic imaging technology. Background technique [0002] Existing fluorescence microscopic imaging can be divided into scanning microscopic imaging based on point-scanning imaging according to the imaging method, and light-sheet microscopic imaging using CCD to detect and image after irradiating fluorescence with a thin beam on the side; in addition, the existing Fluorescence microscopy imaging can be divided into single-photon excitation and two-photon excitation according to the fluorescence excitation conditions. Two-photon excitation is also called multi-photon excitation. Two-photon excitation refers to the fact that fluorescent molecules can absorb two long-wavelength Photons, after the excitation lifetime, emit photons with shorter wavelengths, so two-photon excitation can use light waves with longer wavelengths than single-photon excitation to excite fluorescence, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 杨鑫惠辉田捷董迪马喜波
Owner INST OF AUTOMATION CHINESE ACAD OF SCI
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