Quick clam species distinguishing method

A clam and species technology, which is applied to measuring devices, material analysis through optical means, instruments, etc., can solve the problems of difficult operation, unintuitive detection results, and long time consumption, and achieve convenient operation, accurate and reliable identification results, less time effect

Active Publication Date: 2015-06-10
ZHEJIANG WANLI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0011] Although the above methods are accurate and widely used, they have disadvantages such as difficult operation, long time-consuming, and unintuitive detection results.

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment 1

[0036] Embodiment 1, the experimental materials of this embodiment are 4 kinds of clams of the genus Meretrix collected in the southeast coast of my country, which are respectively clams (collected from Rudong sea area, Nantong, Jiangsu, latitude and longitude are 32 ° 30 'N, 121 ° 11 'E), ax Meretrix meretrix (collected from the Cangnan sea area of ​​Wenzhou, Zhejiang Province, latitude and longitude 27°33′N, 120°36′E), clam meretrix (collected from the sea area of ​​Donghai Island, Zhanjiang, Guangdong Province, latitude and longitude 21°04′N, 110°20′E), Meretrix meretrix (collected from the sea area of ​​Yingpan, Beihai, Guangxi, latitude and longitude 21°27′N, 109°26′E), the shell length is 3-7cm.

[0037] The implementation steps are as follows:

[0038] 1. Live dissection of clams: Clean the 4 kinds of live clams to be inspected, place them in the dissection tray, cut off the anterior adductor muscle with a scalpel, slowly break apart the two shells, and then cut off the p...

Embodiment 2

[0052] Example 2, in June 2012, a kind of clam (locally called "triangle clam") was collected from Zhanggang Town, Changle City, Fujian Province (25 ° 54' 32.78 "N, 119 ° 39' 04.53 "E), its shape and shell color There are certain differences with other species of Meretrix genus. In order to identify Changle "Triangle clam" accurately, two methods of microscopic observation of tentacles at the mouth edge of water inlet and AFLP molecular marker technology were used to study.

[0053] Microscopic observation of tentacles on the mouth of the water inlet pipe observed the tentacles on the mouth of the water inlet pipe of 4 "triangular clams", which took 15 minutes, combined with Figure 5 As shown, the results show that the tentacles on the mouth edge of the "triangle clam" are all scattered in clusters, and the top is like a cauliflower-like branch, which is similar to the figure 2 Meretrix meretrix has the same tentacles at the mouth of the water inlet, so the "triangle clam" ...

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Abstract

A quick clam species distinguishing method is characterized by comprising the following steps of 1 performing clam vivisection, 2 producing an oral siphon peristomial tentacle sheet, 3 performing microscopic observation, and 4 confirming species, namely confirming the clam species according to morphological characteristics of oral siphon peristomial tentacles. Compared with the prior art, The quick clam species distinguishing method has the advantages that the quick clam species distinguishing method utilizes oral siphon peristomial tentacle microscopic observation, is accurate in detection, low in technological difficulty and simple in operation steps and enables a result to be visual and accurate, and especially the total experimental consumption of the quick clam species distinguishing method is obviously lower than those of a traditional chondriosome (COI) gene sequencing method and an AFLP labeling method.

Description

technical field [0001] The invention relates to a method for identifying clam species. Background technique [0002] Meretrix species are one of the most variable species among bivalve molluscs. Due to the influence of genetics and environment, there are great differences in shell shape and shell color pattern. There are great controversies on issues such as taxonomy, taxonomy, and fauna analysis. Chinese scholars also have great differences in the definition of the species of Meretrix. [0003] Zhuang Qiqian's "Chinese Animal History" (Beijing: Science Press, 2001: 229-236.) involves molluscs in the bivalve class Clamidae, and the species of the genus Meretrix distributed in my country are classified as Meretrix (M.meretrix) , Meretrix meretrix (M.lusoria) and clam meretrix (M.1amarckii) 3 species. [0004] "Seashells of China" by Qi Zhongyan (Beijing: Ocean Press, 2004:306); "Illustrated Handbook of Marine Shellfish in China" by Zhang Suping (Beijing: Ocean Press, 2008:3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84
Inventor 董迎辉林志华姚韩韩包永波何琳
Owner ZHEJIANG WANLI UNIV
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