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Lignin-degrading enzyme regulator and application thereof

A technology of lignin degrading enzymes and regulators, applied in the field of microorganisms, can solve problems such as single effect, and achieve the effects of good induction and incentive effect, good degradation effect, and improved degradation efficiency

Active Publication Date: 2015-06-17
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, many inducers have been found, especially metal and non-metal inducers. The research on metal inducers has found that metal ions such as Mn, Mo, Cu, Co, Cd, Hg, Fe, Al have a great effect on lignin degrading enzymes. The non-metallic inducers are mainly aromatic compounds such as oxygen, lignocellulose, reedyl alcohol, paraquat, p-coumaric acid, dihydroxybenzoic acid and guaiacol, but all the studies are on single or A few kinds of metal or non-metallic mixtures are used to induce lignin degrading enzymes, and their effects have certain limitations and singleness. Therefore, it is the future development direction and prospect to study the coordinated induction of inducer mixture systems, and it is also a current research hotspot.

Method used

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  • Lignin-degrading enzyme regulator and application thereof
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Examples

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Effect test

Embodiment 1

[0030] A lignin degrading enzyme regulator of the present invention comprises a regulator of manganese peroxidase and lignin peroxidase (H-1), and a laccase regulator (H-2).

[0031] Wherein the preparation method of H-1 is:

[0032] Glucose, KH 2 PO 4 , MgSO 4 , CaCl 2 , ZnSO 4 、H 2 BO 3 , NaCl, Tween 80, hydrogen peroxide with a concentration of 10mmol / L, trace excitation element mixture A (trace excitation element mixture A includes 5×10 -3 g / L MnSO 4 and 0.6×10 -2g / L FeSO 4 ) were mixed, and the volume was adjusted to 100 ml with acetic acid buffer to obtain the H-1 regulator, the pH of the H-1 regulator was 5 (pH 4.5-5.5 can be implemented).

[0033] In the H-1 regulator, the concentration of glucose is 1.0g / L, KH 2 PO 4 The concentration is 1.47×10 -2 mol / L, MgSO 4 The concentration is 2.03×10 -3 mol / L, CaCl 2 The concentration is 6.8×10 -4 mol / L, ZnSO 4 The concentration is 0.19g / L, H 2 BO 3 The concentration of NaCl is 0.01g / L, the concentration of ...

Embodiment 2

[0042] The application of the lignin-degrading enzyme regulator of Example 1 of the present invention in regulating the secretion of lignin-degrading enzymes in fungi, the specific application method includes the following steps:

[0043] (1) Cultivate Phanerochaete chrysosporium:

[0044] 1.1. Pick Phanerochaete chrysosporium, and culture it in slant culture medium for 5-7 days. Wherein the slant medium adopts potato agar medium (PDA medium). The formula of the PDA medium is: 1000ml potato juice, 20g sucrose, 20g agar, adjust the pH to 5.0, and sterilize at 120°C for 20min.

[0045] 1.2, the bacterial classification on the slant medium is inoculated on the PDA solid plate medium (the formula of the PDA solid plate medium is the same as the PDA medium formula of step 1.1), and when the thalline on the PDA solid medium is covered with the flat plate, use Punch out 4 pieces of 9mm 2 Bacteria flakes of the same size were put into PDA liquid medium (the formula of PDA liquid me...

Embodiment 3

[0061] (1) According to the method of embodiment 2, Phanerochaete chrysosporium is domesticated and cultivated:

[0062] (2) Inoculate the domesticated Phanerochaete chrysosporium into the following fermentation media with an inoculum size of 2.5v / v%:

[0063] Fermentation medium 1: sawmill sawdust (the quality of pure sawmill sawdust accounts for 70% of the total solid content), and the moisture content of the fermentation substrate is 85%.

[0064] Fermentation medium 2: rice straw (the mass of pure rice straw accounts for 70% of the total solid content), and the moisture content of the fermentation substrate is 85%.

[0065] Fermentation medium 3: wheat straw (the mass of pure wheat straw accounts for 70% of the total solid content), and the moisture content of the fermentation substrate is 85%.

[0066] Fermentation medium 4: garden organic plant solid waste (the quality of pure garden organic plant solid waste accounts for 70% of the total solid content), and the moistur...

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PUM

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Abstract

The invention discloses a lignin-degrading enzyme regulator and an application thereof. The lignin-degrading enzyme regulator is added by two steps in an aerobic composting process. The lignin-degrading enzyme regulator comprises a manganese peroxidase and lignin peroxidase regulator and a laccase regulator, wherein the manganese peroxidase and lignin peroxidase regulator comprises mixed liquid A of Tween 80, hydrogen peroxide and trace stimulation elements; the laccase regulator comprises mixed liquid B of Tween 80, 1,2-dimethoxybenzene and trace stimulation elements. The lignin-degrading enzyme regulator can be used for regulating secretion of lignin-degrading enzyme in fungi and has the advantages of good promotion effect, simple, economic and practical technology and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a regulator of lignin degrading enzymes and its application. Background technique [0002] Lignin is primarily a by-product of agriculture, the wood hydrolysis industry, and the paper industry, and is also present in municipal organic solid waste. Due to the large amount of lignin produced and not fully utilized, it has become an environmental pollutant, and a large amount of lignin produced in other fields has also polluted the environment to varying degrees at various levels. Lignin belongs to the phenylpropane polymer structure, which is a very complex and stable chemical structure and is not easy to degrade. Usually, only by changing the stable structure of lignin can it be widely degraded and utilized. At the same time, lignin is also the main and key refractory organic matter in the process of aerobic composting, and its degradation is through the oxidation reaction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
Inventor 胡天觉曾光明张长徐梓一赖萃
Owner HUNAN UNIV
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