Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Establishment method of iron overload mouse model corresponding to thalassemia pesaro risk classification

A risk classification, mouse model technology, applied in the biological field, can solve problems such as adverse reactions, patient death, transplant rejection or recurrence, and achieve the effect of ensuring dose, simple operation, and avoiding gastrointestinal irritation.

Inactive Publication Date: 2018-08-21
SOUTH CHINA NORMAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Excessive doses of busulfan and cyclophosphamide used in conditioning regimens can cause adverse reactions leading to patient death; insufficient doses can cause graft rejection or relapse

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishment method of iron overload mouse model corresponding to thalassemia pesaro risk classification
  • Establishment method of iron overload mouse model corresponding to thalassemia pesaro risk classification
  • Establishment method of iron overload mouse model corresponding to thalassemia pesaro risk classification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The establishment of a level I iron overload mouse model corresponding to the Pesaro risk classification of thalassemia includes the following steps:

[0023] Take healthy C57BL / 6 mice, male, 8 weeks old, weighing 19-23 g. The animals were reared at 23±2°C and 55±5% relative humidity, and the light / dark cycle was set to 12h / 12h. Regular feed was given every day, free food and water, and bedding was changed 2-3 times a week. Intraperitoneal injection of iron dextran (Fe content 100 mg / ml), 1 g iron element / kg body weight, once / week, for a total of 1 week. The experiment was repeated three times.

[0024] The degree of liver enlargement in mice was quantitatively analyzed using liver index (ratio of liver weight to body weight) and liver weight increase ratio [(liver index of mice in each group - liver index of control group) / liver index of control group*100]. The results in Table 1 show that the liver weight of mice increased by 18.7% after 1 week of iron dextran admi...

Embodiment 2

[0029] The establishment of the iron overload mouse model of level II corresponding to the Pesaro risk classification of thalassemia includes the following steps:

[0030] Take healthy C57BL / 6 mice, male, 8 weeks old, weighing 19-23 g. The animals were reared at 23±2°C and 55±5% relative humidity, and the light / dark cycle was set to 12h / 12h. Regular feed was given every day, free food and water, and bedding was changed 2-3 times a week. Intraperitoneal injection of iron dextran (Fe content 100 mg / ml), 1 g iron element / kg body weight, 1 time / week, administration for 2-4 weeks. The experiment was repeated three times

[0031] The results in Table 1 show that after 2-4 weeks of iron dextran administration, the liver weight of mice increased by 50.7-81.9%, and the weight increased by more than 50%. It can be judged that the mice reached the standard of human hepatomegaly exceeding 2 cm in the Pesaro risk classification. .

[0032] figure 1 The results showed that the liver ti...

Embodiment 3

[0036] The establishment of a level III iron overload mouse model corresponding to the Pesaro risk classification of thalassemia includes the following steps:

[0037] Take healthy C57BL / 6 mice, male, 8 weeks old, weighing 19-23 g. The animals were reared at 23±2°C and 55±5% relative humidity, and the light / dark cycle was set to 12h / 12h. Regular feed was given every day, free food and water, and bedding was changed 2-3 times a week. Intraperitoneal injection of iron dextran (Fe content 100 mg / ml), 1 g iron element / kg body weight, once a week, for 5-8 weeks. The experiment was repeated three times

[0038] The results in Table 1 show that after 5-8 weeks of iron dextran administration, the liver weight of mice increased by 92.3-135.7%, and the weight increased by more than 50%. It can be judged that the mice reached the level of human hepatomegaly greater than 2 cm corresponding to the Pesaro risk classification at this time. standard.

[0039] figure 1 The results showed th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for establishing an iron-overload mouse model corresponding to thalassemia Pesaro risk classification. The method comprises the following steps: injecting an iron-containing preparation to the abdominal cavity of a laboratory mouse, wherein the dosage is 1g / kg of the mouse, injecting the iron once a week, wherein the iron comprises Fe<2+> and Fe<3+>; establishing I-grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for one week; establishing II grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for 2-4 weeks; establishing III-grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for 5-8 weeks. The administration interval of the invention is in accordance with the pathological process of the hepatomegaly and hepatic fibrosis induced by the iron overload caused by repeated transfusion of the thalassemia patient, simultaneously, the mouse acute chalybeate poisoning death caused by the large-dose iron administration in a short time or the overlong mouse molding time caused by overlong iron administration time can be avoided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing an iron overload mouse model corresponding to the Pesaro risk classification of thalassemia. Background technique [0002] Thalassemia (thalassemia for short) is an anemia with globin peptide chain (α, β chain) synthesis disorder, which belongs to autosomal genetic disease. Because the early cases were all from the Mediterranean region, it was called thalassemia or thalassemia. Later, it was gradually discovered that this disease is widely distributed in tropical and subtropical regions of the world. The provinces and regions south of the Yangtze River in my country are also high-incidence areas of the disease, especially Guangdong, Guangxi, and Hainan. According to WHO estimates, about 345 million people in the world are carriers of thalassemia, including 80 to 90 million carriers of beta-thalassemia. The birth rate of children with severe tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/721A61K31/7016A61K31/295
Inventor 李续娥石俊敏陶宇晨张琪玥刘启发
Owner SOUTH CHINA NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com