Bt proteins with pesticidal activity against mythimna separata and application thereof

A technology of insecticidal activity and bt protein, applied in the fields of application, insecticide, biocide, etc., can solve the problems of lagging research and development of armyworm control and less attention, and achieve the effect of delaying the generation of insecticide resistance

Pending Publication Date: 2015-07-01
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Compared with the use of Bt to control cotton bollworm, the development of its re...

Method used

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  • Bt proteins with pesticidal activity against mythimna separata and application thereof
  • Bt proteins with pesticidal activity against mythimna separata and application thereof
  • Bt proteins with pesticidal activity against mythimna separata and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1 Protein extraction

[0049] 1) Extraction of Cry1Ab, Cry1Ac, and Cry2Ab proteins

[0050] Cry1Ab, Cry1Ac, and Cry2Ab proteins were all expressed in Bacillus thuringiensis, and were extracted using 1 / 2LB liquid medium and repeated dissolving methods. For specific operations, see: "Enzyme System for Solid Culture of C. Compare" article.

[0051]2) Extraction of Cry1Bb, Cry1Be, Cry9Aa, Cry9Eb, Cry9Ee, and Vip3A11 proteins Cry1Bb, Cry1Be, Cry9Aa, Cry9Eb, Cry9Ee, and Vip3A11 proteins were all expressed in Escherichia coli, and extracted by LB medium, broken and centrifuged, and the specific operation See: "Comparison of enzyme systems for solid cultures of C. conchiformis and Phanerolea chrysosporium". However, due to the different optimal expression conditions of each protein, through repeated exploration, it was found that: Cry1Bb had the highest expression level in the soluble fraction at 18°C ​​with a final IPTG concentration of 1.0mmol / L, and the insoluble fraction ...

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Abstract

The invention provides Bt proteins with pesticidal activity against mythimna separata, and an application thereof. The protein is sourced from bacillus thuringiensis, and is at least one selected from Cry1Ab protein, Cry1Bb protein, Cry1Be protein, Cry2Ab protein, Cry9Aa protein, Cry9Eb protein and Cry9Ee protein. The Bt proteins with high toxicity against mythimna separata assist in filling the lack of mythimna separata controlling resources in the current market. Also, the above proteins provide a novel gene source for the preparation of anti-mythimna separata transgenic crops. The proteins can be used in controlling pest drug resistance against Cry1A products, and in delaying the resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to several Bt proteins with insecticidal activity against oriental armyworm and their applications. Background technique [0002] Oriental armyworm (Mythimna separata) belongs to Lepidoptera, Noctuidae, is a typical long-distance migratory pest, and seriously threatens the food production security in my country. There are three main characteristics of its hazards. First, it occurs in a wide range and harms many generations. In addition to Xinjiang, all parts of the country are affected by it, and there may be 4 to 5 large-scale migratory hazards every year. In other countries in Asia and Australia, damage often occurs, and disasters erupt. The second is that there are many types of crops and tissues that are endangered. Eastern armyworm can harm hundreds of kinds of major cereal crops such as corn, wheat and rice, as well as cotton, beans, vegetables, etc., because it can bite off the...

Claims

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Application Information

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IPC IPC(8): C07K14/325A01N47/44A01P7/04
CPCA01N47/44C07K14/325
Inventor 张杰杨素娟耿丽丽束长龙宋福平彭琦梁影屏
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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