Electricity-force stimulating cell culture apparatus

Abstract

The invention provides an electricity-force stimulating cell culture apparatus, which comprises a cell cultivator and a signal generator, wherein the cell cultivator comprises a cultivator base; a conductive film is arranged on the cultivator base; an electricity-force stimulating electrode plate is arranged above the cell cultivator, and comprises an electricity-force stimulating electrode base on the upper part, a conductive layer on the middle part and a piezoelectric stimulator on the lower part; and a positive electrode and a negative electrode of the signal generator are respectively connected with the conductive layer on the electricity-force stimulating electrode plate and the conductive film on the cell cultivator. When cell cultivation is carried out, electric pulse stimulation can be provided for cells and a culture medium in the cell cultivator; after the piezoelectric stimulator of the electricity-force stimulating electrode plate is electrified, micro-vibration can be generated under the action of current, and the force stimulation equivalent to peripheral cell peristalsis in a living body is provided for the cells. The electricity-force stimulating cell culture apparatus is capable of really simulating the cell stress environment in the living body; and an environment which is relatively close to cell growth environment in the living body is provided for in vitro culture of the cells.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine, cell engineering and tissue engineering, and in particular relates to an electric-force stimulating cell culture device capable of electrically and force-stimulating the cell growth process. Background technique [0002] In modern biomedicine, with the wide application of human implants and tissues and organs in clinical medicine, in vitro cell culture has gradually become an important research method in biomedicine and tissue engineering research. The degree of coincidence between the in vitro culture environment and the actual environment of cell growth in vivo determines the authenticity of cell research results. [0003] Cells in living organisms communicate mainly through bioelectrical and chemical signals, and electrical stimulation is the basic condition for cells to transmit information, produce division, differentiate and migrate. The electric field can not only provide the pulse v...

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Problems solved by technology

[0004] China ZL201010243578.1 discloses a shear force-electrical combined stimulation cell culture device, which uses a signal generator to provide electrical stimulation to the cell culture device, and uses a flat flow chamber to provide shear force stimulation. The flat flow chamber is equipped with a fluid Inlet and outlet, the fluid is provided by upstream and downstream liquid storage bottles and peristaltic pumps. The structure of the device is complex, and fluid flow is used to provide shear force stimulation. It is difficult to control the size and uniformity of the stimulation force, especially the uniformity of the stimulation force. The stimulation force of each part cannot be guaranteed to be consistent

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