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Sperm collecting method for rat

A technology of semen collection and semen collection instrument, applied in the field of rat semen collection, can solve the problems of large damage to animals and difficult implementation of electric stimulation operation for small animals, and achieve the effects of reducing the number of use, reducing the experimental cost and simple operation.

Inactive Publication Date: 2015-07-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of animal semen collection mainly uses electrical stimulation, but electrical stimulation is more harmful to animals, and it is not easy to implement electrical stimulation operations for small animals (such as rats)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Fifteen 18-week-old healthy male rats were selected for the experiment.

[0019] The model of the semen collection device used: a battery-operated personal vibrator (Multicept, Rungsted, Denmark).

[0020] The invention provides a rat semen collection method, which comprises the following steps: first wipe the external genitalia of a male rat with a moistened absorbent cotton ball to stimulate ejaculation; One centrifuge tube, one tweezers, one pipette gun, one constant temperature water bath, and one holding bottle. The semen collection method uses the non-anesthesia holding method. The specific operation steps are as follows:

[0021] 1) Put the male rat head inward into the holding bottle, turn the bottle so that the back of the rat is facing down and the abdomen is facing up, exposing the genitals to realize the holding of the rat;

[0022] 2) After the rat is kept in step 1), carefully scrub the glans of the rat's penis with a sterile cotton ball moistened with ...

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Abstract

The invention discloses a sperm collecting method for a rat and belongs to the biological technical fields. The sperm collecting method for the rat comprises the following steps: 1, maintaining rat in a maintaining bottle; 2, sterilizing apparatus and penis of the rat; 3, using wet cotton ball for wiping genitalia for stimulating the ejaculation, collecting the seminal fluid; 4, using a vibration sperm collecting instrument for stimulating and collecting the seminal fluid. The sperm collecting method for the rat is simple, quick and repeatable in operation, gentle in operation for rat and has no damage for meeting the animal protection requirement; the rat is quiet and comfortable during the collecting process and anesthesia is not needed. The rat sperm collected by the method can be used for artificial insemination for rats, the sperm is stored in a refrigeration mode for the reproductive toxicology search for giving full play to the reproduction efficiency of the male rat and being in favour of the storage of the genetic gene of rat; the use number of male rat in experiment is reduced and the experiment cost is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for collecting sperm in rats. Background technique [0002] In the process of animal experimental research, especially in the process of animal artificial insemination and reproductive toxicology research, animal sperm has become a necessary material for experiments, and how to obtain experimental animal sperm has become a problem that must be solved. At present, the semen collection method of animals mainly uses the method of electrical stimulation, but the electrical stimulation is relatively harmful to animals, and it is not easy to implement the electrical stimulation operation for small animals (such as rats). At the same time, the rat is a typical experimental animal. Therefore, it is of great scientific significance to use a suitable method to collect sperm in rats. The semen collection method that the present invention adopts has not found the same or s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/02
CPCA61D19/021
Inventor 杨世华王歌黄群山杨增明仇思恩刘晓星康平
Owner SOUTH CHINA AGRI UNIV
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