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Molecular labeled primer resistant to brown planthopper gene Bph28(t) and marking method and application thereof

A molecular marker and brown planthopper-resistant technology, applied in the field of molecular genetics, can solve the problems of resistance level and prolonging the duration of resistance, loss of brown planthopper, etc., and achieve the effect of shortening the breeding cycle, clear position and clear selection target

Active Publication Date: 2015-07-08
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the easy variation of BPH biotypes, rice varieties carrying a single resistance gene lost their resistance to BPH in a short period of time, while the resistance level and extension of rice varieties with multiple resistance genes Resistance lasts longer

Method used

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  • Molecular labeled primer resistant to brown planthopper gene Bph28(t) and marking method and application thereof
  • Molecular labeled primer resistant to brown planthopper gene Bph28(t) and marking method and application thereof
  • Molecular labeled primer resistant to brown planthopper gene Bph28(t) and marking method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) F 2 Population construction and phenotyping

[0033] (1) Li et al. (2006) identified resistance to brown planthopper from the transgenic progeny of Guangxi common wild rice (harvested to the wild rice nursery of Guangxi University) and screened for resistance to brown planthopper biotypes 1 and 2, Bangladesh, Mekong (Vietnam ) Jiulongjiang (Vietnam) and Pante Naga (India) all had broad-spectrum, high-resistance antigen W2183. The offspring Bph28(t) monogenic line transduced with this resistance source was used as the resistant parent, and the cultivated rice Huanghuazhan was used as the susceptible parent to cross, and self-crossed to construct F 2 Resistance segregation population 161 strains, each F 2 A single plant obtains the corresponding F by selfing 2：3 family lineage.

[0034] (2) Adopt seedling stage inoculation treatment to parents, F 2：3 Families were tested for insect resistance. To ensure that the parent and F 2： 3Each family in the group grows ...

Embodiment 2

[0057] Validation of Molecular Markers

[0058] 1. Materials and methods

[0059] 1.1 Materials

[0060] Negative varieties: insect-susceptible varieties Huanghuazhan (from Rice Research Institute of Guangdong Academy of Agricultural Sciences), Baimao (Japan), Teqing 2 (from Rice Research Institute of Guangdong Academy of Agricultural Sciences), 9311 (from Anhui Quanyin Hi-Tech Seed Co., Ltd. Company), Nipponbare (Japan), TN1 (International Rice Research Institute), Bph28(t) monogenic line × Huang Huazhan hybrid offspring 13 non-insect-resistant materials, 19 in total.

[0061] Positive varieties: W2183 transgenic offspring, Bph28(t) monogenic line × Huanghuazhan hybrid offspring 17 insect-resistant materials.

[0062] Molecular marker primer: Xue17

[0063] 1.2 Method

[0064] The method for extracting rice genomic DNA and performing PCR amplification of genomic DNA with Xue17 primers is the same as in Example 1.

[0065] 2. Results

[0066] The DNA of 36 different samp...

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Abstract

The invention discloses a molecular labeled primer resistant to brown planthopper gene Bph28(t) and a marking method and application thereof. By means of the F2 generation obtained through hybridization and selfing of a monogenic line (male) and Huanghuazhan (female) of the generation Bph28(t) generated through transformation of the Guangxi wide rice resistance resource W2183, resistance identification and molecular genetic linkage analysis are carried out on various families of F2:3 respectively, and a molecular marker Xue17 resistant to the brown planthopper gene Bph28(t) is obtained. A filial generation genome DNA containing a Bph28(t) parent is amplified through the molecular marker. If an amplification fragment of 191bp can be amplified, it represents that the generation contains a major gene locus Bph28(t) resistant to brown planthopper. By detecting the marker, the resistance to the brown planthopper of rice Bph28(t) monogenic line generation materials can be detected, operation is easy, the accuracy rate is over 95%, and the labeled primer can be used in selective breeding of rice varieties resistant to the brown planthopper and screening of resistance gene resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and in particular relates to a molecular marker primer for the brown planthopper-resistant gene Bph28(t), a marker method and application thereof. Background technique [0002] Brown planthopper is one of the typical piercing-sucking monophagous rice pests. There are different degrees of occurrence in rice-producing areas in China every year. When rice is severely damaged by brown planthoppers, the phenomenon of planthopper fires will appear in the field. Practice has shown that breeding resistant and resistant varieties is the most economical and effective method in integrated control. Discovering and identifying new BPH-resistant genes and understanding their genetic mechanisms are the key to cultivating long-lasting resistant rice varieties. [0003] So far, 33 BPH-resistant genes have been identified and named, and some identified BPH-resistant genes have been mapped to rice chromosomes wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 李容柏薛艳霞邱永福刘芳覃宝祥陈曙
Owner GUANGXI UNIV
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