Structure of gel container, and preparation method thereof

Abstract

The invention discloses a structure of a gel container. The gel container comprises a front preparing sheet, a back preparing sheet, and a sealing adhesive tape; the four corners of the front preparing sheet are provided with four cylindrical embossments which are used for fixing with the back preparing sheet; the two sides and the lower edge of the front preparing sheet are provided with a plurality of welding points which are used for welding shaping with the back preparing sheet; the four corners of the back preparing sheet are provided with four cylindrical grooves which are used for fixing with the front preparing sheet; the lower edge of the back preparing sheet is provided with a hollow groove which is taken as a gel electrode preformed hole; the external of the hollow groove is sealed with the sealing adhesive tape; the external walls of the front preparing sheet and the back preparing sheet are provided with raised marker lines which facilitate observation of electrophoresis position states for users. The invention also discloses a preparation method of the gel container with the structure. According to the structure, the internal walls of the font preparing sheet and the back preparing sheet are coated with polyvinylidene chloride coating with excellent coating performance and oxygen resistance, so that problems, such as protein migration and oxygen interference, of plastic moulds in gel injection processes are solved effectively, plastic moulds are used for replacing glass moulds for gel injection, it is convenient for batch injection of gel, and injection cost is reduced.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to the preparation of biochemical experiment consumables, more specifically, to a structure of an electrophoretic gel container and a preparation method thereof. Background technique [0002] Gel electrophoresis is a routine biochemical laboratory technique for separating protein and nucleic acid molecules. It uses the size sieving capacity of the gel medium to separate charged biomolecules of different sizes through the gel medium with a three-dimensional mesh structure under an electric field. This phenomenon is also known as the molecular sieve effect . [0003] Polyacrylamide gel is currently the most commonly used gel medium in laboratories. It has the advantages of high resolution, electrical neutrality, and good reproducibility, and can be made into gels with various pore sizes. Therefore, polyacrylamide gel Gel electrophoresis (PAGE) has become the most commonly used experimental...

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Problems solved by technology

[0004] Glass molds are generally used to prepare gels in laboratories, but glass molds have disadvantages such as fragile, expensive, weak plasticity, and not suitable for mass gel preparation.

[0005] Although the use of plastic molds to pour gels has the advantages of convenience, economy, and suitability for large-scale pouring of gels, it may lead to a decrease in the band resolution of high-molecular-weight proteins during electrophoresis (this may be due to the gelation of high-molecular-weight proteins in contact with plastic materials). The migration speed on the surface of the gel is faster than the migration speed in the gel, causing the protein bands to tail), in addition, the presence of oxygen will also inhibit the polymerization of acrylamide monomers, affecting the quality of the gel (the interior of the glass mold The facade is flatter than the plastic mold, leaving a small gap, so the interference of oxygen is relatively small and can be ignored)

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