Structure of gel container and its preparation method

Abstract

The invention discloses a structure of a gel container, which includes a front sheet, a rear sheet and a sealing tape. There are four cylindrical protrusions on the four corners of the front sheet, which are used to fix the rear sheet; the two sides of the front sheet and the There are multiple welding points on the lower edge, which are used to weld and shape the rear sheet; there are 4 cylindrical grooves on the four corners of the rear sheet, which are used to fix the front sheet; there is a hollow groove on the lower edge of the rear sheet, which is used for As a reserved hole for the gel electrode, the hollow groove is sealed with sealing tape; there are raised marking lines on the front and rear facades, which are used to facilitate the user to observe the position of the electrophoresis. The invention also discloses a preparation method of the gel container with the above structure. The invention effectively solves the problem of protein migration and oxygen interference in the plastic mold filling process by coating the polyvinylidene chloride coating with good oxygen barrier performance on the front and rear surface of the gel container, so that Plastic molds can replace glass molds for gel casting, which facilitates batch gel casting and reduces casting costs.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to the preparation of biochemical experiment consumables, more specifically, to a structure of an electrophoretic gel container and a preparation method thereof. Background technique [0002] Gel electrophoresis is a routine biochemical laboratory technique for separating protein and nucleic acid molecules. It uses the size sieving capacity of the gel medium to separate charged biomolecules of different sizes through the gel medium with a three-dimensional mesh structure under an electric field. This phenomenon is also known as the molecular sieve effect . [0003] Polyacrylamide gel is currently the most commonly used gel medium in laboratories. It has the advantages of high resolution, electrical neutrality, and good reproducibility, and can be made into gels with various pore sizes. Therefore, polyacrylamide gel Gel electrophoresis (PAGE) has become the most commonly used experimental...

AI Technical Summary

Problems solved by technology

[0004] Glass molds are generally used to prepare gels in laboratories, but glass molds have disadvantages such as fragile, expensive, weak plasticity, and not suitable for mass gel preparation.

[0005] Although the use of plastic molds to pour gels has the advantages of convenience, economy, and suitability for large-scale pouring of gels, it may lead to a decrease in the band resolution of high-molecular-weight proteins during electrophoresis (this may be due to the gelation of high-molecular-weight proteins in contact with plastic materials). The migration speed on the surface of the gel is faster than the migration speed in the gel, causing the protein bands to tail), in addition, the presence of oxygen will also inhibit the polymerization of acrylamide monomers, affecting the quality of the gel (the interior of the glass mold The facade is flatter than the plastic mold, leaving a small gap, so the interference of oxygen is relatively small and can be ignored)

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