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Method for inducing settlement and metamorphosis of capitulum mitella linnaeus larvae with juvenile hormone-III

A technology of juvenile hormone and Venus larvae, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, drug delivery, etc., can solve the problem of tortoise-footed Venus larvae attachment metamorphosis, etc., and achieve significant induction effect, stable effect, and low cost Effect

Active Publication Date: 2015-07-22
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although tortoise-footed Venus larvae can be cultivated on a large scale indoors at present, it is necessary to break through the problem of inducing tortoise-footed Venus larvae to attach and metamorphose under artificial conditions in order to realize the artificial breeding of tortoise-footed larvae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Use dimethyl sulfoxide to help solubilize according to the ratio of juvenile hormone-Ⅲ / dimethyl sulfoxide of 1 / 25 (w / v), add ethanol to prepare a preparation containing juvenile hormone-Ⅲ 0.1mg / mL,- Store at 20°C. Add filtered seawater before use to prepare seawater containing juvenile hormone-Ⅲ in 0.2 mg induction. Culture and collect Venus larvae, introduce Venus larvae into induced seawater and soak for 8 hours, then replace with fresh seawater. After 4 days of induction culture, Venus larvae can complete metamorphosis, and the metamorphosis rate reaches 92.4%.

[0015] The conditions for using the induced seawater are as follows: the water temperature is 25° C., the salinity is 28, the pH is 7.8, and the induction time is 8 hours.

Embodiment 2

[0017] Use dimethyl sulfoxide to help solubilize according to the ratio of juvenile hormone-Ⅲ / dimethyl sulfoxide of 1 / 25 (w / v), add ethanol to prepare a preparation containing juvenile hormone-Ⅲ 0.1mg / mL,- Store at 20°C. Before use, add filtered seawater to prepare 100mL of seawater containing juvenile hormone-Ⅲ in 0.4 mg induction. Culture and collect Venus larvae, introduce 1,000 Venus larvae into induced seawater and soak for 12 hours, then replace with fresh seawater. After 4 days of induction culture, Venus larvae can complete metamorphosis, and the metamorphosis rate reaches 95%.

Embodiment 3

[0019] Use dimethyl sulfoxide to help solubilize according to the ratio of juvenile hormone-Ⅲ / dimethyl sulfoxide of 1 / 25 (w / v), add ethanol to prepare a preparation containing juvenile hormone-Ⅲ 0.1mg / mL,- Store at 20°C. Before use, add filtered seawater to prepare 400mL of seawater containing juvenile hormone-Ⅲ in 0.3 mg induction. The Venus larvae were cultured and collected, and 3200 Venus larvae were introduced into induced seawater for soaking for 6 hours, and then replaced with fresh seawater. After 5 days of induction culture, a total of 2870 larvae metamorphosed, and the metamorphosis rate reached 89.6%.

[0020] The preparation formula and the application method provided by the invention have a good effect on inducing attachment metamorphosis of the tortoise-foot Venus larvae.

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PUM

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Abstract

The invention relates to the technical field of aquaculture, in particular to a preparation and an application method for inducing settlement and metamorphosis of capitulum mitella linnaeus larvae with juvenile hormone-III. The invention adopts the technical scheme that a preparation for inducing the settlement and the metamorphosis of the capitulum mitella linnaeus larvae is prepared in a water body by the following steps of carrying out solubilization with dimethyl sulfoxide according to a weight-to-volume ratio of the juvenile hormone-III to dimethyl sulfoxide as 1 / 25 (w / v), adding alcohol to prepare the preparation containing 0.1mg / mL juvenile hormone -III, and storing at -20 DEG C. Filtered sea water is added to prepare inducing sea water before use. The preparation is simple to operate, low in cost, stable in action and significant in inducing effect, and a metamorphosis rate can reach 86%. The method presents appropriate concentration, treatment time and inducing opportunity for application of the preparation and is a good method for inducing the settlement and the metamorphosis of the capitulum mitella linnaeus larvae, and the metamorphosis rate of the larvae is up to 95%.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a preparation and an application method for using juvenile hormone-Ⅲ to induce attachment and metamorphosis of Venus tortoise larvae. Background technique [0002] Turtle feet ( Capitulum mitella Linnaeus belongs to Arthropoda, Crustacea, Cirripedia, Thoracica, Pedunculata, Scalpellomorpha, refers to the turtle foot of Pollicipidae Genus ( Capitulum ), named for its shape resembling a turtle's foot, also known as stone masonry, bergamot, turtle's foot, pen holder, etc. It is mainly distributed in the crevices of rocks in the intertidal zone of tropical and subtropical seas. Turtle feet have high nutritional value and medicinal value. At present, the turtle feet on the domestic market rely on natural sea areas to catch them, which is difficult and has caused certain damage to the habitat of turtle feet. In order to rationally develop and utilize Tortoise foot resources, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K31/351A61P43/00
Inventor 饶小珍林岗
Owner FUJIAN NORMAL UNIV
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