Detection method and application of soybean cyst nematode resistance-related map-5037snp marker
A soybean cyst nematode, map-5037snp technology, applied in the biological field, can solve the problems of not being able to meet the requirements of line-resistant breeding, difficulty in success, long time, etc., achieve remarkable breeding effects, shorten breeding time, and improve selection efficiency
Active Publication Date: 2018-01-02
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Due to the genetic complexity of SCN resistance genes and the diversity of soybean cyst nematode physiological races, it takes a long time to cultivate varieties resistant to soybean cyst nematode disease by using traditional disease resistance phenotype identification and breeding methods. Moreover, it is difficult to succeed, and it is no longer able to meet the current development of line-resistant breeding.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0040] Example 1, Map-5037SNP site is a single nucleotide polymorphism associated with soybean cyst nematode No. 3 physiological race resistance
[0041]The Map-5037 single nucleotide polymorphism site is located at the 2716th position of the Glyma.08g103800 gene nucleotide sequence (sequence 1), and its basic information is shown in Table 1.
[0042] Table 1. Basic information of Map-5037SNP single nucleotide polymorphism site
[0043]
[0044] 2. Association analysis between Map-5037SNP genotype and soybean cyst nematode race 3 disease resistance
[0045] 1. Detection of resistance of soybean varieties to race 3 of soybean cyst nematode
[0046] The soybean varieties in Table 2 and Table 3 were all inoculated with soybean cyst nematode race 3 in the field disease nursery (the pathogen is soybean cyst nematode race 3) and in the greenhouse. In the field, a completely randomized block test design was adopted, with three repetitions. The field planting row width is 0.65 m...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a detection method of a Map-5037SNP mark relevant to the soybean cyst nematode resistance and the application of the detection method. The invention provides the application of polymorphism substances or genotypic substances detecting Map-5037SNP sites in a soybean gene group to identification or auxiliary identification of the resistance of soybeans to No. 3 physiological strains of the soybean cyst nematode. The invention also provides the application of the polymorphism substances or the genotypic substances detecting the Map-5037SNP sites in the soybean gene group to preparation of the product for identifying or assisting in identifying the resistance of the soybeans to the No. 3 physiological strains of the soybean cyst nematode. Experiments prove that the Map-5037SNP sites are screened out by the detection method, and the resistance to the No. 3 physiological strains of the soybean cyst nematode can be provided for breeding materials at the early stage of varieties through detection on the gene types at the Map-5037SNP sites, so that the selecting efficiency of disease resistant varieties in the breeding process is greatly improved, the breeding time is shortened, and the breeding effect of the varieties resistant to the soybean cyst nematode is obvious.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a detection method and application of soybean cyst nematode resistance-related Map-5037 SNP markers. Background technique [0002] Soybean cyst nematode (Soybean Cyst Nematode, SCN) has the characteristics of wide distribution, heavy damage, multiple transmission routes, wide host range, and long survival time. It is a soil-borne disease that is extremely difficult to control. A devastating blow. At present, the disease has a tendency to spread and become more serious, which has seriously restricted soybean production. Practice has proved that cultivating and planting disease-resistant varieties is the most effective way to prevent and control diseases. Due to the genetic complexity of SCN resistance genes and the diversity of soybean cyst nematode physiological races, it takes a long time to cultivate varieties resistant to soybean cyst nematode disease by using traditional disease...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 邱丽娟李英慧史学晖
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI