Method for preparing ocean composite peptide biological fertilizer through solid state fermentation
A technology of solid-state fermentation and compound peptides, which is applied in the direction of fertilizer mixture, preparation of organic fertilizers, bio-organic part treatment, etc., can solve the problems of ignoring the use value of waste, the added value of products cannot be increased, and cannot be used, so as to improve soil fertility and Crop quality, promotion of agricultural production and income, efficient absorption and utilization
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Embodiment 1
[0016] Example 1: 400 g of soybean meal and 1000 g of ground low-value marine biological raw material were prepared into a solid-state fermentation base material. KH 2 PO 4 2g, 20g of sucrose and water are mixed to prepare 1000g of nutrient solution, and the pH value is adjusted to 7.0. Mix the solid-state fermentation base material and nutrient solution evenly to make a solid-state fermentation medium, sterilize at 115°C for 30 minutes, and then cool it down for later use.
[0017] Preparation of Bacillus seed solution: Inoculate the nutrient broth liquid medium and shake on a shaker at 32°C for 24 hours to logarithmic phase.
[0018] Preparation of Actinomycetes Seed Solution: Inoculate the nutrient broth liquid medium and shake it on a shaker at 32°C for 36 hours to the logarithmic phase.
[0019] Inoculate the Bacillus and Actinomycetes seed liquids in the logarithmic phase according to 10% of the weight of the above-mentioned solid-state fermentation medium, stir them ...
Embodiment 2
[0021] Example 2: 500 g of soybean meal and 1000 g of ground low-value marine biological raw material were prepared into a solid-state fermentation base material. KH 2 PO 4 6g, 45g of sucrose and water are mixed to make 1500g of nutrient solution, and the pH value is adjusted to 7.0. Mix the solid-state fermentation base material with the nutrient solution to make a solid-state fermentation medium, sterilize at 115°C for 30 minutes, and cool down for later use.
[0022] Preparation of Bacillus seed solution: Inoculate the nutrient broth liquid medium and shake on a shaker at 32°C for 24 hours to logarithmic phase.
[0023] Preparation of Actinomycetes Seed Solution: Inoculate the nutrient broth liquid medium and shake it on a shaker at 32°C for 36 hours to the logarithmic phase.
[0024] Inoculate the bacillus and actinomycete seed liquids in the logarithmic phase according to 5% of the weight of the above-mentioned solid-state fermentation medium, stir them evenly and then...
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