Method for preparing ocean composite peptide biological fertilizer through solid state fermentation

A technology of solid-state fermentation and compound peptides, which is applied in the direction of fertilizer mixture, preparation of organic fertilizers, bio-organic part treatment, etc., can solve the problems of ignoring the use value of waste, the added value of products cannot be increased, and cannot be used, so as to improve soil fertility and Crop quality, promotion of agricultural production and income, efficient absorption and utilization

Inactive Publication Date: 2015-07-29
青岛诺海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These raw materials cannot be used for aquatic food processing due to many reasons such as active tissue enzymes in the body and low meat-to-bone ratio. Some raw materials are even ignored and discarded as waste.
At the same time, in the process of aquatic product processing, a large amount of processing waste is often produced. The large amount of protein, sugar, lipid and small molecular active substances contained in these waste materials have not been fully recycled, and the added value of the product cannot be improved. waste, and also lead to serious pollution of marine and terrestrial environments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: 400 g of soybean meal and 1000 g of ground low-value marine biological raw material were prepared into a solid-state fermentation base material. KH 2 PO 4 2g, 20g of sucrose and water are mixed to prepare 1000g of nutrient solution, and the pH value is adjusted to 7.0. Mix the solid-state fermentation base material and nutrient solution evenly to make a solid-state fermentation medium, sterilize at 115°C for 30 minutes, and then cool it down for later use.

[0017] Preparation of Bacillus seed solution: Inoculate the nutrient broth liquid medium and shake on a shaker at 32°C for 24 hours to logarithmic phase.

[0018] Preparation of Actinomycetes Seed Solution: Inoculate the nutrient broth liquid medium and shake it on a shaker at 32°C for 36 hours to the logarithmic phase.

[0019] Inoculate the Bacillus and Actinomycetes seed liquids in the logarithmic phase according to 10% of the weight of the above-mentioned solid-state fermentation medium, stir them ...

Embodiment 2

[0021] Example 2: 500 g of soybean meal and 1000 g of ground low-value marine biological raw material were prepared into a solid-state fermentation base material. KH 2 PO 4 6g, 45g of sucrose and water are mixed to make 1500g of nutrient solution, and the pH value is adjusted to 7.0. Mix the solid-state fermentation base material with the nutrient solution to make a solid-state fermentation medium, sterilize at 115°C for 30 minutes, and cool down for later use.

[0022] Preparation of Bacillus seed solution: Inoculate the nutrient broth liquid medium and shake on a shaker at 32°C for 24 hours to logarithmic phase.

[0023] Preparation of Actinomycetes Seed Solution: Inoculate the nutrient broth liquid medium and shake it on a shaker at 32°C for 36 hours to the logarithmic phase.

[0024] Inoculate the bacillus and actinomycete seed liquids in the logarithmic phase according to 5% of the weight of the above-mentioned solid-state fermentation medium, stir them evenly and then...

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Abstract

The invention relates to a method for preparing an ocean composite peptide biological fertilizer through solid state fermentation. The method comprises the following steps: mixing soybean meal and a ground low-value marine animal raw material at the proportion of (2 to 8):10 till the obtained mixture I is uniform; mixing the mixture I with a nutrient solution at the proportion of 1:(0.5 to 2.5) till the obtained mixture II is uniform; adjusting the initial pH of the mixture II to be 7.0 to 7.5; respectively inoculating bacillus of which the mass concentration is 5 to 10 percent and an actinomyce seed solution according to weight ratio; carrying out solid state fermentation at 25 to 40 DEG C for 2 to 8 days; carrying out water extracting, filtering and concentration after a culture is obtained after fermentation, so as to obtain an ocean composite peptide biological leaf fertilizer; drying the ocean composite peptide biological leaf fertilizer directly; smashing the dried ocean composite peptide biological leaf fertilizer to obtain the ocean composite peptide biological water soluble fertilizer. The ocean composite peptide biological fertilizer prepared according to solid state fermentation has the advantages that the raw materials are high in availability and low in cost; pollution emission is avoided; various agriculture beneficial functions of growth promotion, water conservation, fertility enhancement, bacteriostasis and the like can be performed.

Description

technical field [0001] The invention relates to the field of marine biological fertilizers, in particular to a method for preparing marine compound peptide biological fertilizers by using low-value marine biological resources and soybean meal mixed solid-state fermentation. Background technique [0002] Low-value marine biological resources refer to raw materials rich in aquatic protein, mainly low-value fish such as anchovies and jack mackerel. These raw materials cannot be used for aquatic food processing due to many reasons such as active tissue enzymes in the body, perishability, and low meat-to-bone ratio. Some raw materials are even ignored for their use value and discarded as waste. At the same time, in the process of aquatic product processing, a large amount of processing waste is often produced. The large amount of protein, sugar, lipid and small molecular active substances contained in these waste materials have not been fully recycled, and the added value of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05F17/00
CPCY02W30/40
Inventor 牟海津程雯邢坤
Owner 青岛诺海生物科技有限公司
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