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Method for constructing multi-parental population by using soybean ms1 male sterility

A technology of male sterility and ms1, which is applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of time-consuming, laborious, tedious, and difficult hybridization, and achieve a wide range of separation types, save labor costs, and enrich The effect of genetic diversity

Inactive Publication Date: 2015-08-12
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, soybean builds a multi-parent population. The number of parents is less than 5, and the method of single-cross, double-cross, and three-cross is used for hybridization. The number of parents is more than 6, and the method of diallel hybridization is generally used. As the number of parents increases, the difficulty of hybridization will also increase. Large, and artificial detasseling and artificial pollination of soybeans are very cumbersome, time-consuming and labor-intensive, and the success rate of hybridization is only 20-40%

Method used

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  • Method for constructing multi-parental population by using soybean ms1 male sterility
  • Method for constructing multi-parental population by using soybean ms1 male sterility
  • Method for constructing multi-parental population by using soybean ms1 male sterility

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1. Using soybean ms1 male sterile crosses to construct a hex population with a broad genetic base

[0026] 1. Collect 2500 soybean ms1 male sterile seeds.

[0027] 2. Select parents that meet the target traits

[0028] In this embodiment, 63 parents satisfying the target traits were selected (see Table 1).

[0029] Table 1 63 parents of target traits

[0030]

[0031]

[0032]

[0033] Note: D-high protein, protein content>45.0%; Y-high oil, oil content>21.5%; JD-pod more; Z-plant good; T-special type; DL-big grain; G-high yield.

[0034] Note: Varieties with serial numbers 1-14 are obtained through WDD codes, and WDD represents the number of foreign soybean varieties in the National Crop Germplasm Bank; varieties with serial numbers 15-47 are obtained through ZDD codes, and ZDD represents the National Crop Germplasm Bank of China Soybean variety numbers; varieties with serial numbers 48-63 are all recorded in "Huanghuaihai Soybean Germplasm Improvem...

Embodiment 2

[0042] The genetic diversity analysis of embodiment 2, hex population

[0043]1. Group planting

[0044] The hex population obtained in Example 1 was planted in 800 rows with a row spacing of 50 cm and a plant spacing of 15 cm.

[0045] Select 60 soybean resource materials (referred to as "provincial resources", see Table 2) that can represent the main genetic traits of soybean from Hebei Provincial Agricultural Biological Resource Conservation Center (formerly Hebei Provincial Crop Germplasm Resource Bank) as controls, and carry out the hex population For diversity analysis, 2 rows were planted for each resource material.

[0046] Table 2 60 Soybean Resource Materials in Hebei Province (Provincial Capital)

[0047]

[0048]

[0049] Note: Varieties with serial numbers 1-17 and 20-53 are obtained through ZDD numbers, and ZDD represents the number of Chinese soybean varieties in the National Crop Germplasm Bank; varieties with serial numbers 54-60 are all recorded in "H...

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Abstract

The present invention discloses a method for constructing a multi-parental population by using soybean ms1 male sterility, and provides a method for constructing a multi-parental population by using soybean ms1 male sterile hybridization. The method comprises: (1) collecting at least 2500 soybean ms1 male sterile seeds; (2) collecting at least 3 soybean parental seeds meeting the target trait, and equally mixing; (3) uniformly mixing the soybean ms1 male sterile seeds collected in the step (1) and the mixed seeds obtained in the step (2) according to a ratio of 1:3-6; (4) planting the seeds obtained in the step (3), and harvesting sterile plant seeds at a mature stage in a mixed manner; (5) planting the seeds obtained in the step (4), and harvesting all plant seeds at a mature stage in a mixed manner; and (6) planting the seeds obtained in the step (5), and harvesting all fertile plant seeds at a mature stage in a mixed manner so as to obtain the multi-parental population. With the method of the present invention, the cumbersome processes such as artificial emasculation and artificial pollination are eliminated, and the labor cost can be saved by 90% compared with the conventional artificial hybridization constructing method.

Description

technical field [0001] The invention relates to a method for constructing a multi-parent population by using ms1 male sterility. Background technique [0002] my country's soybean resources are very rich, but the utilization rate of soybean resources in the cultivation of new varieties is only about 1%, which leads to the narrow genetic basis of soybean varieties and the difficulty of breeding breakthrough varieties. The lack of breeding methods utilizing soybean resources is the key. [0003] The main breeding methods of soybean include conventional cross breeding method, chemical mutagenesis breeding method, radiation mutagenesis breeding method and so on. Among them, 95% of soybean varieties are bred by conventional cross-breeding methods, 3% are bred by chemical mutagenesis, and few varieties bred by other methods. Conventional cross-breeding is the main method of cultivating new varieties, and it is the most mature method of soybean breeding in both theoretical resear...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 张孟臣赵双进闫龙杨春燕张梅申赵青松
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI