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American ginseng molecule ID and identification method

A molecular ID card and identification method technology, applied in the field of identification of Chinese medicinal materials source varieties, can solve problems such as difficulty in character identification, and achieve the effect of wide applicability and accurate identification

Inactive Publication Date: 2015-08-12
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the similar appearance of related species such as Panax ginseng and counterfeit products, it is difficult to identify traditional traits

Method used

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  • American ginseng molecule ID and identification method
  • American ginseng molecule ID and identification method
  • American ginseng molecule ID and identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 is used to illustrate the identification method of American ginseng medicinal raw materials

[0032] 1) From Muling City and Ning'an City in Heilongjiang, Ji'an City, Tonghua City, Jingyu County, Fusong County in Jilin Province, Xinbin County and Qingyuan County in Liaoning Province, Huairou District in Beijing, Wendeng City in Shandong Province, Anguo Pharmaceutical City in Hebei Province, Bozhou in Anhui Province A total of 40 parts of American ginseng P.quinquefolium were collected in Yaoshi, Canada and Wisconsin, the United States, the dried roots of confounded product P. ginseng, A. tetraphylla or A. astricta, and Angelica dahurica A. Dahurica or Hangbaizhi A.dahurica var.formosana sample 12 parts, take 20mg medicinal material respectively, grind on MM400 ball mill (Germany Retsch), and extract the total DNA with the plant genome DNA kit of Tiangen Biochemical Technology (Beijing) Co., Ltd. .

[0033] 2) PCR amplification

[0034] The primer sequence is...

Embodiment 2

[0046] The identification of embodiment 2 American ginseng decoction pieces

[0047] Using the same method as in Example 1 for identification, the difference is that American ginseng decoction pieces (American ginseng root slices) are used as samples to extract DNA for identification. As a result, the American ginseng molecular identity card can also be specifically detected. And sequence comparison found that the ITS2 sequence (SEQ ID No.1) of the American ginseng decoction pieces DNA contained ATCACTCCTTTGCGGGAGTCGAGGCGG.

Embodiment 3

[0048] The identification of embodiment 3 American ginseng powder

[0049] Using the same method as in Example 1 for identification, the difference is that American ginseng powder is used as a sample, DNA is extracted, and identification is carried out. The result can also specifically detect the molecular ID card of American ginseng, and because American ginseng ITS2 has no intraspecific variation, it can be Realize the identification of American ginseng powder.

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PUM

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Abstract

The invention relates to an American ginseng molecule ID which contains a nucleotide sequence represented as the SEQ ID No.1. The American ginseng molecule ID and an identification method therewith can accurately separate American ginseng from a fake product thereof, which is similar with true American ginseng in appearance. The method is wider in adaptability, can be used for detecting any samples of which DNA can be extracted, so that the American ginseng molecule ID can achieve quick and accurate identification of raw plants, medicines, seeds, seedlings, powders and adulterants of the American ginseng.

Description

technical field [0001] The invention belongs to the technical field of identification of source varieties of Chinese medicinal materials, and in particular relates to a molecular ID card of American ginseng and an identification method. Background technique [0002] American ginseng is the dried root of Panax quinquefolium L., an Araliaceae plant. It has the effects of invigorating qi and nourishing yin, clearing heat and promoting body fluid. embolism. Panax ginseng and Panax ginseng belong to the genus Panax genus Araliaceae, and their properties are similar, so it is difficult to distinguish them by traditional identification methods. There is a big difference between the two in terms of medicinal properties and efficacy. American ginseng is cool in nature, nourishes yin and reduces fire, nourishes qi and promotes body fluid, calms and relieves fever; while ginseng is mild in nature, nourishes vitality, restores pulse and solidifies, and excites the central nervous syste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/158
Inventor 韩建萍宋经元王丽丽陈士林
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI