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Method for detecting collagen enzymolysis activity of trepang cathepsin

A cathepsin and activity detection technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of low enzymatic hydrolysis efficiency of collagen, meet the requirements of enzyme activity evaluation, and facilitate the acquisition of raw materials

Inactive Publication Date: 2015-08-26
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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Problems solved by technology

At present, the above-mentioned types of enzymes generally have the disadvantage of low enzymatic hydrolysis efficiency of collagen. Therefore, the development of new high-efficiency collagenases has important application value.

Method used

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  • Method for detecting collagen enzymolysis activity of trepang cathepsin
  • Method for detecting collagen enzymolysis activity of trepang cathepsin

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Embodiment

[0014] Example: refer to Figure 1 to Figure 2 , a method for detecting collagen enzymatic hydrolysis activity of sea cucumber cathepsin, comprising: using sea cucumber cathepsin to evaluate the effect of enzymatic hydrolysis on the diameter of depressions produced by enzymatic hydrolysis of the collagen gel surface. Enzyme activity can be evaluated by enzymatic hydrolysis diameter or area, and semi-quantitative results can be obtained. It can meet most of the enzyme activity evaluation requirements and is relatively accurate. The detection method has the following steps:

[0015] 1) Collagen gel production: using common collagen-rich food raw materials in daily life as production materials, after boiling, fat removal, filtration to remove impurities, pH value adjustment, solid content measurement, and pouring plate coagulation steps, the production into collagen gel;

[0016] 2) Preparation of protease solution: Dissolve the protease standard at the concentration of 10mg / m...

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Abstract

The invention discloses a method for detecting collagen enzymolysis activity of trepang cathepsin, wherein the method comprises: adopting the trepang cathepsin to carry out enzymolysis effectiveness evaluation to the sunken diameter generated by enzymolysis on the surface of collagen gel. An enzymolysis diameter or area is adopted to carry out evaluation to enzymatic activity, and a semiquantitative result can be obtained. The method for detecting the collagen enzymolysis activity of the trepang cathepsin can meet a large proportion of enzymatic activity evaluation requirement, and is relatively accurate. Therefore, the method for detecting the collagen enzymolysis activity of the trepang cathepsin has the advantages of being suitable for trepang protease, able to carry out semiquantitative evaluation to the collagen enzymolysis activity of the trepang cathepsin, and convenient for obtaining raw materials.

Description

technical field [0001] The invention relates to a method for detecting enzymolysis activity, in particular to a method for detecting collagen enzymolysis activity of sea cucumber cathepsin. Background technique [0002] Collagen is a structural protein of body tissue, and its many physiological functions have been systematically studied and widely recognized. At present, the domestic collagen industry is developing rapidly. With the rapid development of the collagen industry, the demand for protease, the key production factor in the collagen enzymatic hydrolysis process, is also increasing. At present, the commonly used collagenases can be divided into plant proteases (such as bromelain, papain, etc.), animal proteases (such as trypsin, pepsin, etc.), microbial proteases, etc. from the source. At present, the above-mentioned types of enzymes generally have the disadvantage of low enzymatic hydrolysis efficiency of collagen. Therefore, the development of new high-efficiency ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/37
Inventor 王天明肖金星郑刚杨水兵余海霞
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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