Tissue sealant in which collagen and fibrin are mixed, and method for preparing same

A fibrin and fibrinogen technology, applied in peptide/protein components, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of lack of physical properties and high biodegradation rate of fibrin glue, and improve the quality and reliability, high viability, good growth rate effect

Inactive Publication Date: 2015-09-09
SEWON CELLONTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when compared to sealants using synthetic materials, fibrin glue has the disadvantages of its lack of physical properties and its relatively high rate of biodegradation

Method used

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  • Tissue sealant in which collagen and fibrin are mixed, and method for preparing same
  • Tissue sealant in which collagen and fibrin are mixed, and method for preparing same
  • Tissue sealant in which collagen and fibrin are mixed, and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Comparison of physical properties between the present invention and prior art

[0062] In order to verify the physical properties of the present invention, the maximum stress, gel strength and tensile strength were measured using a physical property meter.

[0063] 1. Sample preparation

[0064] 1) In the prior art, Greenplast products are used.

[0065] 2) For the components of the present invention, Greenplast's dried fibrinogen and thrombin were dissolved in aprotinin solution and added calcium solution, respectively. Here, a thrombin solution is mixed with a 3% collagen solution. The resulting solution was loaded into a two-way syringe.

[0066] 3) For the measurement of physical properties, each sample was put into a cylindrical mold (Φ12X 15mm) to make a shape.

[0067] 2. Measurement of physical properties

[0068] 1) Physical property instrument: Rheometer (CR-500DX, Sun scientific rheometer)

[0069] 2) Test items: maximum stress (N), gel strength (g-cm),...

Embodiment 2

[0075] Comparison of degradability (long term / short term) between products of the invention and prior art

[0076] In order to verify the degradability of the compositions of the invention, fibrin glue products and materials were tested for degradability for a predetermined period of time.

[0077] 1. Degradability (short term)

[0078] 1) Sample preparation

[0079] - In the prior art, Greenplast products are used.

[0080] - For the composition of the invention, Greenplast's dried fibrinogen and thrombin were dissolved in aprotinin and added calcium solution, respectively. Here, a thrombin solution is mixed with a 3% collagen solution. The resulting solution was loaded into a two-way syringe.

[0081] - For the measurement of physical properties, each sample was put into a cylindrical mold (Φ8X 5mm) to make a shape.

[0082] 2) Processing conditions for degradability verification

[0083] - Confirm both conditions for the solvent. The condition of using only DEME medi...

Embodiment 3

[0097] Electron Microscopy Analysis of the Invention

[0098] Observation of the structure of the composition of the present invention by electron microscopy

[0099] 1. Sample preparation

[0100] - For the preparation of the composition, a fibrinogen solution of Greenplast and a thrombin solution / calcium solution containing collagen are prepared. The concentration of the collagen solution was 3% (weight / volume).

[0101] - For electron microscopy, each prepared solution was applied to a two-way syringe for distribution on trays.

[0102] 2. Method

[0103] - The composition of the invention is critically dried and then observed with an electron microscope.

[0104] -Critical point drying of the composition was performed by treating ethanol in a critical point dryer (Hitachi, HCP-2).

[0105] - The sample for electron microscope observation was cut and gold plated, followed by observation with SEM (Hitachi, S3500).

[0106] - Electron microscopy analysis was performed a...

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Abstract

The present invention relates to a tissue sealant in which collagen and fibrin are mixed and to a method for preparing same. To this end, the method of the present invention comprises the steps of: mixing a first material using fibrinogen and aprotinin; mixing a second material using thrombin, calcium chloride, and collagen; and preparing a third material by mixing the first material and the second material. The tissue sealant prepared by the method may supplement weaknesses, i.e. strength and degradability, of the fibrin sealant which is currently available in the commercial marketplace. Further, the tissue sealant of the present invention is cytophilic and activates blood platelets contained in blood so as to induce tissue regeneration. Thus, quality and reliability of products can be significantly improved so as to satisfy various needs of consumers who are users, thereby presenting good image.

Description

technical field [0001] The present invention relates to a tissue sealant that mixes collagen and fibrin, and a method of making the tissue sealant, and more specifically, the invention resides in supplementary strength and degradability, which are indicated as fibrin adhesives in the market agent's weakness. That is, the present invention relates to a tissue sealant that, when the tissue sealant has affinity with cells, activates platelets contained in blood to induce tissue regeneration, so that the quality and reliability of the product can be remarkably improved to meet the requirements as various needs of consumers. Background technique [0002] As is well known, medical sealants have been applied in various fields ranging from surgical adhesion and attachment to hemostasis, and have a long history. Since medical sealant materials are applied directly to human tissue, biocompatible materials need to be used. Since the medical sealant material can basically flow into b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L33/12A61K38/36
CPCA61L26/0033A61K38/39A61L26/0042A61K38/363A61L26/0052C08L89/06A61K2300/00A61K38/36A61L33/12A61B17/00491A61M5/19A61L2400/04A61B2017/00495
Inventor 柳志喆吕世根金壮勋李准根徐东三张晶皓
Owner SEWON CELLONTECH CO LTD
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